胚胎干细胞体外定向诱导分化研究进展  

The study progress of induced commitment and differentiation of ES cells in vitro

作　　者：宋晓平[1] 窦忠英[1] 

机构地区：[1]西北农林科技大学家畜生殖内分泌与胚胎工程农业部重点开放实验室,陕西杨陵712100

出　　处：《西北农林科技大学学报（自然科学版）》2003年第2期161-165,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"973"项目 (G19990 5 43 0 1-5 ) ;国家自然科学基金资助项目 (3 9970 3 63 )

摘　　要：胚胎干细胞是由早期内细胞团分离的一种多潜能细胞 ,在体外分化抑制培养时 ,可保持未分化状态而无限增殖。一旦撤除分化抑制因素 ,或添加适当的诱导剂 ,ES细胞可分化为内胚层、中胚层和外胚层的多种类型的特化细胞。文章综述了体外定向诱导 ES细胞分化为造血细胞、心肌细胞、神经细胞、脂肪细胞、胰岛细胞、内皮细胞、上皮细胞、成骨细胞、软骨细胞和黑素细胞等的途径和方法。Embryonic stem cells are pluripotent cells derived from the inner cell mass of early blastrocyts that can be propagated indefinitely in the primitive undifferentiated state under in vitro differentiation inhibited culture.Under appropriate conditions embryonic stem cells have been shown to differentiate in vitro to multiple cell types of endoderm,mesoderm and ectoderm.This paper reviews the pathways of ES cells in vitro being committed and differentiated to hematopoietic cells,cardiomyocytes,neural cells,adipocytes,pancreatic islets,endothelial cells,epithelia,osteoblasts,chondroblast and melanocytes.

关 键 词：胚胎干细胞 体外分化 诱导剂 定向诱导 

分 类 号：Q813.1[生物学—生物工程] Q954.4