小麦品种及单基因系与叶锈菌互作的组织学和超微结构研究  

Histopathological and ultrastructural studies on interactions between wheat leaf rust and wheat cultivars Lovrin 10 and Jimai 3 as well as their corresponding resistance genes Lr26 and Lr3Bg

作　　者：黄国红[1,2] 王瑶[1] 康振生[1] 魏国荣[1] 朱之堉[3] 李振岐[1] 

机构地区：[1]西北农林科技大学植物保护学院 [2]河北农业大学植物保护系,河北保定071001 [3]河北农业大学植物保护系

出　　处：《西北农林科技大学学报（自然科学版）》2003年第2期45-54,共10页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金资助项目 (3 0 0 70 496) ;国家杰出青年基金资助项目 (3 0 12 5 0 3 1) ;陕西省科技攻关项目 (2 0 0 1K0 2 -G4)

摘　　要：应用荧光显微镜、微分干涉显微镜和电镜技术 ,比较研究了洛 10和冀麦 3号及其相应的单基因系L r2 6和 L r3Bg接种小麦叶锈菌后的组织病理学和超微结构特征。洛 10和 L r2 6的组织学表现极其相似 ,呈典型的早期过敏性坏死反应 ,导致侵染结构败育 ;冀麦 3号和 L r3Bg的组织学表现出一定差异 ,锈菌在 L r3Bg上的发展要快于呈现出慢锈特征的冀麦 3号。超微结构研究表明 ,小麦叶锈菌在洛 10和冀麦 3号上的发育受到了程度不同的抑制 ,表现为锈菌和寄主在细胞和亚细胞水平上有一系列变化。过敏性坏死反应和胼胝质的形成是 2个重要的抗锈机制。The histopathological and ultrastructural features of Lovrin 10 and Jimai 3 as well as Lr26 and Lr3Bg were examined by using fluorescent microscopy,differential interference contrast microscopy and electron microscopy at various times after inoculation with urediospores of wheat leaf rust.Lr26 and Lr3Bg were supposed to be the dominant resistance genes in Lovrin 10 and Jimai3 respectively.Lovrin 10 and Lr26 showed similar histopathological manifestation,exhibiting the typical early hypersensitive reaction with few mycelia observed.While there was some difference between the histopathological manifestation of Jimai3 and Lr3Bg.Jimai3 exhibited the typical features of slow rusting,while Lr3Bg showed the features close to those of susceptible cultivar.Ultrastructural observation indicated that both the rust and host underwent a series of changes at the cellular and subcellular levels.Hypersensitive reaction and callose formation were two important resistance mechanisms.

关 键 词：小麦 品种 单基因系 叶锈菌 超微结构特征 组织病理学 抗病基因 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]