重组人肝细胞生长因子β链体外生物学活性的测定  

Bioactivity assay of recombinant human hepatocyte growth factor-β in vitro

作　　者：常冰梅[1] 牛勃[1] 程牛亮[1] 张悦红[1] 王惠珍[1] 解军[1] 杨涛[1] 

机构地区：[1]山西医科大学生物化学与分子生物学教研室,030001

出　　处：《中国药物与临床》2003年第2期122-124,T002,共4页Chinese Remedies & Clinics

摘　　要：目的　建立一种稳定灵敏的方法用于检测重组人肝细胞生长因子β链 (rhHGF β)对原代培养成年大鼠肝细胞的增生作用。方法　利用噻唑蓝 (MTT)比色方法在多种实验条件下对rhHGF β进行生物学活性检测。结果　对于体外原代培养成年大鼠肝细胞 ,rhHGF β可刺激肝细胞的增生 ,且有剂量依赖效应。 结论　确定了原代培养肝细胞检测rhHGFObjective To work out a stable and sensitive method (as for the cell number,culture time,etc) to detect the effect of recombinant human hepatocyte growth factor-β (rhHGF-β) on the multiplication of primary culture of hepatocytes in adult rats.Methods Bioactivity of rhHGF was assayed under various conditions using MTT.Results rhHGF-β stimulated multiplication of primary adult rat hepatocytes.Conclusion The author sifted out an optimal scheme for detecting the bioactivity of rhHGF-β in primary culture of liver cells.

关 键 词：重组人肝细胞生长因子β链 体外 生物学活性 测定 MTT检测 肝细胞增生 

分 类 号：R346[医药卫生—基础医学]