石墨炉原子吸收法测定血清锰  

Determination of serum manganese with graphite AAS

作　　者：余秋月[1] 王先良[1] 杨永坚[1] 刘浩[1] 胡浩[1] 刘肖文 张良璞 钟平 

机构地区：[1]安徽医科大学公共卫生学院劳动卫生与环境卫生系,合肥230032 [2]安徽省合肥市环境监测站,合肥230031

出　　处：《安徽医科大学学报》2003年第1期40-42,共3页Acta Universitatis Medicinalis Anhui

摘　　要：目的　建立一种血清锰的石墨炉原子吸收分光光度分析方法。方法　将血清和 0 44mol/L的HNO3按 1 9稀释后 ,1 5 0 0 0r/min高速离心 1 0min ,取上清 2 0 μl直接测定。结果　发现灰化温度为 1 30 0℃ ,原子化温度为1 90 0℃ ,净化温度为 2 4 50℃和基底改进剂为 5 μg钯 +3μg硝酸镁最佳 ;平均回收率可达到 99 56 % ;变异系数 (CV)为5 0 % ;特征浓度为 6 376 1 0 pg /0 0 0 4 4A ,检出限为 6 7pg。结论　本方法为一种可信度较高。Objective To set up a new method of detecting the l evel of manganese in serum with the graphite atomic absorption spectroscopy(AAS) . Methods The serum was diluted with HNO 3 and centrifuged 10 minu tes by 15 000 r/min first and the solution above in the tube was going to be determined. Results The optimal temperatures for cineration, atomi zation and clearance were 1 300 ℃, 1 900 ℃ and 2 450 ℃ with the matri x modifier of a unit of 5 μg Pd and 3 μg Mg(NO 3) 2, respectively. The avera ge reclamation rate was 99 56% and the coefficient of variation was about 5%; t he sensitivity was 6 376 10 pg / 0 004 4 A and the minimal level could be detected was 6 7 pg. Conclusion This is a practicable and cre dible method for detection serum Mn with AAS.

关 键 词：石墨炉原子吸收法 测定 血清 锰 变异系数 

分 类 号：R446.112[医药卫生—诊断学]