TDZ诱导花生幼叶的不定芽和体细胞胚发生的组织学观察  

HISTOLOGICAL STUDY ON ADVENTITIOUS BUD FORMATION AND SOMATIC EMBRYOGENESIS OF PEANUT (ARACHIS HYPOGAEA L. ) LEAFLETS

作　　者：林荣双[1] 王庆华[1] 梁丽琨[1] 肖显华[1] 

机构地区：[1]烟台大学生化系,烟台264005

出　　处：《植物研究》2003年第2期169-171,T007,共4页Bulletin of Botanical Research

基　　金：山东省自然科学基金(Y97D16075)

摘　　要：花生实生苗幼叶接种于MS+TDZ 0.2mg/L+NAA 0.4mg/L诱导培养基上经诱导培养,继而转移到无激素培养基MS可获得不定芽和体细胞胚。组织学观察表明,花生不定芽和体细胞胚均起源于愈伤组织表层,不定芽为多细胞起源,而体细胞胚起源于单个胚性原始细胞。体细胞胚的发育经历多细胞原胚、球形胚、心形胚、鱼雷胚和子叶胚等时期发育成小植株。This paper deals with a histological observation of adventitious bud formation and somatic embryogenesis of peanut (Arachis hypogaea L. ) leaflets. Adventitious buds and embryoids developed from the leaflets of 4 - day - old seedlings on MS basai medium supplemented with TDZ 0. 2mg/L and NAA 0.4mg/L, and developed into plantlets after they were transferred onto MS medium without any phytohormone. Histological observations showed that both adventitious buds and somatic embryos developed from the surface of the callus. However, adventitious buds originated from cell mass while somatic embryos originated from one single embryogenic cell. The somatic embryos developed directly into plantlets with roots and buds through multicellular proembryo, globular, heart -shaped, torpedo - shaped and cotyledon stages

关 键 词：花生 幼叶 组织学 不定芽发生 体细胞胚发生 组织培养 植物基因工程 种质改良 培养基 

分 类 号：S565.2[农业科学—作物学]