6-DMAP和胚胎干细胞代数对异种重构卵体外发育的影响(简报)  

EFFECTS OF 6-DIMETHYLAMINOPURINE AND PASSAGES OF EMBRYONIC STEM CELLS ON THE DEVELOPMENT IN VITRO OF INTERSPECIFIC RECONSTRUCTED OOCYTES

作　　者：王鸿[1] 郑瑞珍[2] 廉莉[3] 徐营[3] 安利佳[1] 陈大元[3] 

机构地区：[1]大连理工大学生物工程系,大连116012 [2]中国科学院发育生物学研究所分子发育生物学开放实验室,北京100080 [3]中国科学院动物研究所生殖生物学国家重点实验室,北京100080

出　　处：《实验生物学报》2003年第2期145-148,共4页Acta Biologiae Experimentalis Sinica

基　　金：国家科技部攀登专项(95-专-08);中科院知识创新重大项目(KSCX1-05-01)

摘　　要：核移植技术已经广泛应用于动物克隆,但是克隆动物的成活率仍然很低[1-3].The development of reconstructed cocytes and the survival rate of cloned animal were affected by many factors during nuclear transfer. The genetic constitution and the genetic state of donor nucleus were proposed to be primary factors, which affected the survival rate of cloned animal. In addition, the survival rate of cloned animal might be influenced by nuclear transfer technique itself and passages of donor cells as well as the activation methods of oocytes. We reconstructed oocytes with outbreeding Kunming albino mouse ES cells and enucleated rabbit oocytes, and analyzed the effects of the passages of ES cells and 6-DMAP on the development of interspecific reconstructed cocytes. The interspecific reconstructed ES-ribbit oocytes were activated either by combined two set electric pulses and 6-DMAP or by two set electric pulses alone. The rate of cleavage was significantly higher for the group (86. 2%) treated with 6-DMAP than the group (64.2%,P<0.05) treated with electric pulses only, and the rate of blastocysts was 17.0% and 13.4% respectively, which were not significantly different between two groups. When ES cells that had been passed for 24 and 14 generations were used as donors, the cleavage rates of the reconstructed oocytes were 88. 5% and 82. 1% , respectively (P> 0.05), and the rates of blastolation were 16.7% and 15.4% , respectively (P>0.05). The results show that 6-DMAP increases the cleavage rate of reconstructed oocytes derived from ES cells, and affects slightly the developmental rate of blastocysts. There are no differences when high passage and low passage ES cells are used as nuclear donors.

关 键 词：异种克隆 胚胎干细胞 代数 6-DMAP 核移植 胚胎发育 动物克隆 异种重构卵 

分 类 号：Q953[生物学—动物学]