兔抗人NESTIN抗血清的制备与鉴定(简报)  

PREPARATION AND IDENTIFICATION OF RABBIT-ANTI-HUMAN NESTIN ANTISERUM

作　　者：刘磊[1] 林万敏[1] 林隽姬[1] 刘璇[1] 郭兰萍[1] 徐平[1] 

机构地区：[1]复旦大学生命科学院基因生理学实验室脑研究中心立人实验室,上海200433

出　　处：《实验生物学报》2003年第2期163-166,共4页Acta Biologiae Experimentalis Sinica

摘　　要：巢蛋白(nestin)属Ⅵ类中等纤维蛋白[1].最初在神经系统发育早期发现有该蛋白的表达[2].Nestin belongs to the class Ⅵ intermediate filament family and it is a marker for neural progenitor cells. In this work, the 3'-terminal coding sequence(396bp) of human nestin gene was cloned into pGEX-3X plasmid and introduced into BL21 E. coli cells. The GST-nestin protein was purified with an affinity column. Anti-human nestin antiserum was raised by immunizing a rabbit with the fusion protein. The high specificity of the antibody against human nestin was confirmed by western-blot and immunocytochemistry analysis.

关 键 词：兔抗人NESTIN抗血清 GST融合蛋白 制备 鉴定 巢蛋白 免疫学检测 WESTERN-BLOT 免疫印迹 免疫细胞化学 

分 类 号：R392.1[医药卫生—免疫学]