伯氏疟原虫氯喹敏感株和抗性株虫体食物泡和疟色素形态及形成的比较  

Comparison of formation and morphologic features of digestive vacuoles and haemozoin between chloro-quine-sensitive and chloroquine-resistant strains of Plasmodium berghei

作　　者：陈克强[1,2] 朱淮民[1] 宋关鸿[1] 

机构地区：[1]第二军医大学基础医学部病原生物学教研室 [2]上海交通大学农学院动物科学系,上海201101

出　　处：《第二军医大学学报》2003年第4期387-389,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(39880041).

摘　　要：目的:了解伯氏疟原虫氯喹敏感株(N)和抗性株(RC)红内期虫体在抗药性、致病力和诱导免疫应答方面差异的形态学基础。方法:应用超薄切片和透射电镜技术,比较N株和RC株虫体食物泡和疟色素形态及形成的差异。结果:N株滋养体具有单一的、大型的食物泡,疟色素位于滋养体周边的质膜下;在裂体生殖中,疟色素发生聚合和中心化。RC株滋养体食物泡少见,其中多数虫体形成多个微型单层膜的食物泡样结构;疟色素形成明显少于N株滋养体;而且在裂体生殖中,未见疟色素聚合和中心化。结论:伯氏疟原虫RC株长期在氯喹压力下,形成了微型食物泡样结构的摄食方式,具有与N株疟原虫不同的对游离血红素的解毒机制,这可能是伯氏疟原虫N株和RC株在抗药性、致病力和诱导宿主免疫应答方面差异的基础。Objective: To understand the morphologic foundation for differences in drug-resistance, virulence and immunity between chloroquine-sensitive (N) and chloroquine-resistant (RC) strains of Plasmodium berghei. Methods: The RC strain and the N strain were compared concerning the formation and morphologic features of digestive vacuoles and haemozoin with transmission electron microscope. Results: There was a single large digestive vacuole and multiple micro-single-mem-braned vacuole-like structures in the trophozoites of the N strain, and haemozoins centralized and fused during their schizo-gony were situated under the plasma membrane. Whereas there were few digestive vacuoles in the trophozoites of the RC strain, but with multiple micro-single-membraned vacuole-like structures instead. The RC strain formed obviously less hemo-zoins than that of the N strain and the hemozoins were not centralized and fused during the schizogony. Conclusion: The RC strain forms multiple single-membraned food vacuole-like structures in the trophozoites, and has different mechanism for detoxifying free heme with N strain and the features may be the foundation for the difference in drug-resistance, virulence, immunity between RC strain and N strain of Plasmodium berghei.

关 键 词：伯氏疟原虫 氯喹 敏感株 抗性株 虫体食物泡 疟色素形态 形成 比较 

分 类 号：R382.31[医药卫生—医学寄生虫学]