A型肉毒毒素轻链基因的克隆及其结构分析  

Cloning and sequencing of the light chain gene of botulinus neurotoxin serotype A

作　　者：贾宏丽[1] 赵金红[2] 王景林[1] 康琳[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071 [2]解放军208医院检验科,长春130062

出　　处：《生物技术通讯》2003年第2期116-118,共3页Letters in Biotechnology

摘　　要：以文献报道的A型肉毒毒素基因全序列为标准,设计并合成一对引物,自肉毒梭菌基因组中扩增出肉毒毒素轻链基因片段,并将扩增产物与pGEM-T载体在体外连接,构建测序重组质粒,进行测序和基因结构分析。PCR扩增获得了产物为1364bp的DNA片段,测序结果与DNA数据库对照检索分析证明,此基因片段与GenBank中的A型肉毒毒素LC基因的一致性达99.9%以上,可以认为克隆的基因为A型肉毒毒素LC基因。JA DNA fragment encoding type A Clostridium botulinum light chain(BoNT/A LC)was amplified and cloned into a sequencing plasmid pGEM-T.Cloned gene was sequenced and analyzed.A1364bp DNA fragment was amplified.Recombinant of BoNT/A LC was constructed.Sequence analysis proved that the cloned gene was BoNT/A LC.It is important to clone BoNT/A LC successfully in behalf of expression in Escherichia coli and further study on its function and detection.

关 键 词：A型肉毒毒素 轻链 基因克隆 基因结构 毒性 肉毒梭菌 

分 类 号：R378.83[医药卫生—病原生物学] Q785[医药卫生—基础医学]