声波刺激对中华猕猴桃ATP含量的影响  

Study on effect of ATP content about Actinidia chinensis callus under given sound stimulation

作　　者：阳小成[1] 王伯初[2] 段传人[2] 

机构地区：[1]华南师范大学生命科学学院,广州510631 [2]重庆大学生物工程学院生物力学与组织工程教育部重点实验室,重庆400044

出　　处：《生物技术通讯》2003年第2期123-126,共4页Letters in Biotechnology

基　　金：国家自然科学基金资助项目(39770206)

摘　　要：根据环境应力对植物应激效应的原理,运用声波刺激木质藤本植物中华猕猴桃茎段的愈伤组织,测定ATP含量及其变化情况,并通过与对照组(CK)相应值的比较,研究声波对植物能量代谢的影响。采用单因子实验设计,分别固定作为交变应力的声波的频率或强度这两个参数中的一个,而改变另一个指标的值,对实验材料进行处理,实验结果表明,声波对猕猴桃愈伤组织ATP的含量有着比较明显的增强或抑制的双重效应,适度的声场刺激将有利于提高植物的能量代谢水平,其中,最适的声波频率为1000Hz,而最适声强为100dB左右。More and more attentions have been taken to the effects of environmental stresses on the growth and de-velopment of plant cells and tissues.Making efforts on this field would enhance plant adaptability to varied environ-ments and to implement extensively efficient agricultural technologies.Using callus cultured from stalks of Actinidia chinensis,this study dealed with the effects of sound wave stimulation on plant cell energy metabolize i.e.content of ATP.The results indicated that there occurs bidirectional effects of ATP content on sound field stimulation.The sound intensity of about 100dB and sound frequency of approximate1000Hz are optimal external stresses for the growth and development of A.chinensis.Moderate stresses would be advantageous to A.chinensis.The mechanisms of ATP content effects of sound wave stimulation on A.chinensis callus was comprehensively discussed in the light of cytobiology and molecular biology.Where as the essential mechanisms of biological effects of environmental stresses remains further research.

关 键 词：中华猕猴桃 愈伤组织 声波刺激 ATP含量 环境应力 植物 能量代谢 

分 类 号：Q947.8[生物学—植物学] S663.4[农业科学—果树学]