两性霉素B和5-氟胞嘧啶的体外联合药敏试验  

Susceptibility testing of yeasts to combination of amphotericin B and 5-flucytosine in vitro according to M27-A method

作　　者：陈宏[1] 温海[2] 徐红[2] 廖万清[2] 

机构地区：[1]解放军第421医院皮肤科,广州510318 [2]第二军医大学长征医院皮肤性病科,上海200003

出　　处：《第二军医大学学报》2003年第4期453-454,共2页Academic Journal of Second Military Medical University

基　　金：军队"九五"重点课题资助项目(96Z037).

摘　　要：参照美国国家临床试验标准化委员会(NCCLS)提出的标准,采用棋盘微量稀释法对28株隐球菌和念珠菌进行两性霉素B和5-氟胞嘧啶的体外联合药敏试验。结果显示联合用药时各药物的最低抑菌浓度(minimal inhibitory concentration,MIC)几何均值比单独用药显著降低(P<0.01);两性霉素B和5-氟胞嘧啶的协同相加作用较为明显,而且未发现有拮抗作用,部分抑菌浓度指数(fractional inhibitory concentration index,FICI)的平均值为0.8。提示两性霉素B和5-氟胞嘧啶对致病酵母主要表现为协同相加作用。This article is to study the interaction between amphotericin B and 5-flucytosine to yeasts in vitro. The checkerboard microdilution method was applied to study in vitro interaction according to M27-A method of NCCLS. Twenty-eight strains of yeasts were used for combination study. Compared with the single agents,there were significant reductions in the geometric mean of the minimal inhibitory concentration of the combination(P<0. 01). Synergetic and additive interactions were observed obviously without antagonism. The average value of fractional inhibitory concentration index was 0. 8. Antifun-gal synergy exists between amphotericin B and 5-flucytosine against pathogenic yeasts.

关 键 词：两性霉素B 5—氟胞嘧啶 体外联合用药 药敏试验 抗真菌药物 

分 类 号：R978.5[医药卫生—药品]