Isolation and Preparative Purification for Ginkgolides A and B  

作　　者：韩金玉 王华 常贺英 褚巧伟 

机构地区：[1]School of Chemical Engineering and Technology

出　　处：《Chinese Journal of Chemical Engineering》2003年第2期125-129,共5页中国化学工程学报（英文版）

基　　金：Supported by the Natural Science Foundation of Tianjin(No.993606911).

摘　　要：In this paper a simple preparative method for isolation and purification of ginkgolides A and B was developed,As starting material,a commercially available standardized ginkgo extract (EGb761,containing 24% flavonoid and 6% terpene trilactones) was used,After a pretreatment step,optimized by the uniform design method ,the concentrated intermediate extract with high content of GA and gb(+90%) was separated into the individual terpenes by preparative liquid chromatography eluted with petroleum ether-ethylacetate,Analysis of products was carried out by means of HPLC-ELSD(evaporative light -scattering detector),The results show that ginkgolides A and B are obtained in higher yield and better purity.In this paper, a simple preparative method for isolation and purification of ginkgolides A and B was developed. As starting material, a commercially available standardized ginkgo extract (EGb761, containing 24% flavonoid and 6% terpene trilactones) was used. After a pretreatment step, optimized by the uniform design method, the concentrated intermediate extract with high content of GA and GB (+90%) was separated into the individual terpenes by preparative liquid chromatography eluted with petroleum ether-ethylacetate. Analysis of products was carried out by means of HPLC-ELSD (evaporative light-scattering detector). The results show that ginkgolides A and B are obtained in higher yield and better purity.

关 键 词：ginkgolide A ginkgolide B isolation and purification uniform design preparative liquid chromatog-raphy 

分 类 号：TQ028[化学工程]