纳米金标记法在DNA检测及基因芯片技术中的应用  

Applications of Au Nanoparticles Labeling Method in DNA-detect ion and the Technology of Genechip

作　　者：谷宇[1] 何农跃[1] 

机构地区：[1]东南大学国家教委分子与生物分子电子学重点研究实验室,南京210096

出　　处：《中国药科大学学报》2003年第2期184-189,共6页Journal of China Pharmaceutical University

基　　金：国家高技术研究发展计划 ( 863计划 )项目 ( 10 2 0 1 0 1) ;国家自然科学基金 ( 60 0 710 0 1);江苏省高新技术项目(BG2 0 0 10 10 )~~

摘　　要：近年来DNA介导的纳米金颗粒的组装研究受到广泛重视 ,烷巯基修饰的寡核苷酸可与纳米金共价结合 ,形成的探针可与目的多核苷酸序列杂交 ,纳米金颗粒被可逆组装成超分子结构 ,聚集过程伴有红色到蓝色的颜色变化 ,由此衍生出比色法检测特定DNA序列的新方法。该方法具有极高的选择性和较好的灵敏度 ,与银染法结合更提高了检测的灵敏度 ,可用于斑点杂交、芯片检测等领域。本文对该方法的原理、操作过程及研究进展作一简要介绍。Recently, the research in the DNA mediated assembling of Au nanopartic les has caused wide attention. Mercaptoalkyloligonucleotides c an be covalently bound to Au nanoparticles, which form probes. When these probes were hybridized with th e target polynucleotides sequence, the Au particles are assembled reversibly and form a supermolecular structure. The polymerization process is accompanied by a red to blue color change, providing a novel colorimetric detection technique of sequence specific DNA. This is a highly selective and sensitive method, and th e sensitivity of the detection can be further enhanced by the silver deposition. It is believed that the combination of Au nanoparticles labeling with silver dep osition amplification will find its use in the field of blot hybridization and a rray detection. This article introduced the principle,the process of the operation a nd the research progress of the technique.

关 键 词：纳米金 比色法 银染 DNA检测 

分 类 号：Q819[生物学—生物工程]