在对映体水平上测定土壤中烯效唑的方法  

Determination of Uniconazole in Soil with an Enantioselective Way

作　　者：李朝阳[1] 张智超[1] 刘怡姗[1] 周其林[1] 高如瑜[1] 王琴孙[1] 

机构地区：[1]南开大学元素有机化学研究所元素有机化学国家重点实验室,天津300071

出　　处：《高等学校化学学报》2003年第5期840-842,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学青年基金(批准号:29907002);教育部高等学校骨干教师资助计划资助

摘　　要：目前使用的农药中约25%是手性化合物[1]. 手性农药对映体的生物活性往往有很大差异, 例如常用的三唑类植物生长调节剂烯效唑(Uniconazole), 其S体的活性是R体的7倍[2].A fast and precise method was developed for the determination of the enantiomers of chiral pes-ticidc-uniconazole in soil. Matrix solid-phase dispersion (MSPD) technology was used to extract uniconazole from soil. MSPD conditions were optimized stepwise. The MSPD extract was directly used for quantitative determination of uniconazole by silica-based high performance liquid chromatography(HPLC) with UV detection. The recoveries of uniconazole from three types of soils fortified at three levels of 0. 1, 1, 10 μg/g ranged from 75%-97% with RSDs<7%. On this basis, uniconazole was further isolated from the remainder of MSPD extract by silica-based HPLC and then subjected to chiral HPLC to determine its enan-tiomcric ratio. The determined ERs in the soils spiked with racemic uniconazole agreed sufficiently well with those in the standard solutions within the error limit of the method.

关 键 词：手性农药 烯效唑 对映体比值 手性高效液相色谱 测定 污染物 分析 土壤 

分 类 号：X833[环境科学与工程—环境工程] X132