FT_3、FT_4放射免疫分析(直接法)方法学研究  

Study on the methodology of FT_3 and FT_4 radioimmunoassay (direct method)

作　　者：许叔祥 邹颂海[2] 杨文如 唐国忠[3] 赵夏令[3] 周美英[3] 殷林祥[3] 

机构地区：[1]上海南洋医学放射免疫检测中心,上海200020 [2]上海铁道医学院附属甘泉医院 [3]中国科学院上海原子核研究所,上海201800

出　　处：《核技术》1992年第5期285-290,共6页Nuclear Techniques

摘　　要：自行研制了^(125)I标记的T_3X、T_4X(酰胺类衍生物)。免疫所得抗T_3抗体滴度1:160000,K=1.13×10^(11)l/mol;抗T_4抗体滴度1:6000,K=7.6×10^(10)l/mol。以PR试剂为分离剂,建立一步直接放免测定法。灵敏度FT_3为0.24 pmol/l;FT_4为0.16pmol/l。方法精确、特异、稳定。临床应用结果与英国Amersham Amerlex—M法相关极好。The measurement of FT3 anid FT4 is one of the most direct indicators of thyroid status. A thyronine derivative (T3X) was synthesized and used as the precursor for preparing 125I-T3X and 125I-T4X with high specific activity. The antiserum titres are 1:160000 for T3 and 1:6000 for T4,and the affinity constants (K) are 1.13×1011 I/mol for T3 and 7.6×10101/mol for T4. Using specific precipitating reagent to separate F and B, the one-step direct method of RIA has been established. It is accurate, specific and stable. The sensitivity is 0.24pmol/l for FT3 and 0.16pmol/l for FT4. The direct method and its kits have been used succes-sfully in some clinical laboratory. When compared with Amersham's Amerlex-M kits, the results of both are quite coincidence.

关 键 词：FT3 FT4 PR试剂 放射免疫测定 

分 类 号：R392.11[医药卫生—免疫学]