日本血吸虫雌雄成虫可溶性蛋白组分的双向电泳分析  

Soluble Proteins analysis of male and female Schistosoma japonicum by two-dimensional gelelectrophoresis

作　　者：吴忠道[1] 冯明钊[2] 徐劲[1] 孟玮[1] 阮志燕[1] 余新炳[1] 

机构地区：[1]中山大学中山医学院寄生虫学教研室,广州510089 [2]香港中文大学生物系

出　　处：《热带医学杂志》2001年第2期120-122,共3页Journal of Tropical Medicine

基　　金：国家自然科学基金 (No:30 0 0 70 6 83) ;国家教育部博士点基金 (No :2 0 0 0 45 );广东省自然科学团队研究项目资助

摘　　要：目的　分析日本血吸虫雌雄成虫可溶性蛋白组分。方法　双向电泳法。结果　用双向电泳分析雌雄虫体多肽斑点分别有 310个和 2 90个 ,匹配的蛋白点为 5 1个。日本血吸虫雌雄成虫可溶性蛋白中存在一些共同多肽斑点 ,但不同的多肽斑点较多。结论　日本血吸虫雌雄成虫可溶性蛋白组分存在较大差异 ,值得进一步研究。Complementation of male and female worms is essential for the maturation of adult worms in Schistosoma japonicum . Although several sex specific genes of S.japonicum were identified, the major difference between the male and female worm in protein level have not been studied. In this study, we reported the analysis of the soluble proteins of the male and female worms by two-dimensional gelelectrophoresis. The 2D-gel electrophoresis revealed 310 and 290 polypeptides in female and male respectively. 51 polypeptides were the same as identified by the 2-D analysis programme. This unexpectedly great difference between the male and female worms in protein level requires further studies.

关 键 词：双向电泳 日本血吸虫 雌虫 雄虫 蛋白质 

分 类 号：R383.24[医药卫生—医学寄生虫学]