^(211)At-3H_(11)McAb的免疫活性及体外杀伤效应研究  

A study on the immunoactivity of ~211At -antigastric cancer McAb3H_11 and its injury effect on gastric cancer cells

作　　者：王娟[1] 周懋伦[1] 金建南[1] 张叔渊[1] 刘宁[1] 罗林[1] 王方元[1] 万文徽 董志伟 

机构地区：[1]四川大学原子核科学技术研究所,成都610064 [2]北京肿瘤防治研究所,北京100034

出　　处：《核技术》1992年第11期678-682,共5页Nuclear Techniques

基　　金：国家自然科学基金

摘　　要：以间接标记法首先合成中间体对砹苯甲酸,再通过活化羧基反应联接到3H_(11)McAb上,制得^(211)At-3H_(11)McAb偶联物,放射性比度为18.5—61.1kBq/μg。ELISA法鉴定标记后的^(211)At-3H_(11)McAb的免疫活性与未标记3H_(11)McAb一致。实验表明:^(211)At-3H_(11)McAb对靶细胞杀伤效应高于^(211)At-IgG及Na^(211)At;当靶细胞先与一定量3H_(11)McAb或3G_9McAb(4μg)作用1h,可抑制^(211)At-3H_(11)McAb对靶细胞的杀伤效应,放射性比度不影响杀伤效应。The synthesis of 211 At labelled 3H11 monoclonal antibody was performed by an activation reaction of carboxy via paraastato - benzoic acid intermediate. Its specific radioactivity was 18. 5-61. 1kBq/μg . Its immunoactivity was measured by ELISA method. In vitro binding and cytotoxic experiments showed when the 211At - 3H11McAb concentration is raised, the cytotoxic effect increased and have obvious selectivity. The cytotoxic effect of 211At - 3H11 McAb to target cell M85 was higher than that of 211At - IgG and Na211At . If the M85 cells were cultured with 3H11 McAb or 3G9McAb for 1 h at first, the cytotoxic effect of 211At -3H11McAb was inhibited. The specific radioactivity of 211At - 3H11McAb and cytotoxic effect are also discussed.

关 键 词：放射疗法 免疫疗法 肿瘤 砹同位素 

分 类 号：R817.1[医药卫生—影像医学与核医学]