半导体固体光源诱导荧光检测系统的研制  

Design of an Induced Fluorescence Detector System with Semiconductor Solid State Light Sources as Excitation Sources

作　　者：杨丙成[1] 谭峰[1] 陈令新[1] 关亚风[1] 

机构地区：[1]中国科学院大连化学物理研究所现代分析与微型仪器研究室,大连116012

出　　处：《生命科学仪器》2003年第2期35-37,共3页Life Science Instruments

基　　金：自然科学基金(批准号298270011);科技部十五学仪器攻关课题(批准号011806)资助

摘　　要：采用共聚焦光学结构、半导体激光和发光二极管固体光源为激发光源,研制了一种便携式荧光检测系统.整机采用模块化结构,利用分立的检测池模块,可满足微流量分析及常规分析的需要.采用毛细管电泳技术评价了该检测体系,浓度检测限为0-7～10-9M,线性范围为102～103.A portable fluorescence detection system based on confocal configuration and using semiconductor lasers and light-emitting diode as excitation sources is described. Modulat design was adopted to allow the detection system to be applied for micro-fluidic and conventional analyses by changing suitable detection cells. The detection system was evaluated using capillary electrophoresis (CE). The detection limit of concentration was 10-7~10-9M and the linear dynamic range was 102-103.

关 键 词：荧光检测系统 激光诱导荧光检测 LIF-D 研制 

分 类 号：TH744.4[机械工程—光学工程] O657.34[机械工程—仪器科学与技术]