用细胞内细胞因子染色方法检测HLA-A~*02个体CD8^+T细胞的免疫应答  

The immune response of CD8^+T cells from subjects of different HLA-A~*02 subtype detected by intracellular cytokine staining

作　　者：李若冰[1] 陈红松[1] 李丹[2] 费然[1] 丛旭[1] 范春蕾[1] 魏来[1] 王宇[1] 

机构地区：[1]北京大学人民医院肝病研究所,北京100044 [2]北京大学人民医院血液病研究所HLA实验室,北京100044

出　　处：《中国免疫学杂志》2003年第4期262-265,共4页Chinese Journal of Immunology

基　　金：国家"8 63"项目 (编号 2 0 0 1AA2 1715 1);国家自然科学基金项目 (编号 :3 0 170 0 47);北京大学医学部"985"项目

摘　　要：目的 :用细胞内细胞因子染色方法 (ICCS)检测不同HLA A 0 2个体的CD8+ T细胞对流感病毒多肽的免疫应答状况。方法 :采用SSP法确定HLA A 0 2亚型 ;分离HLA A 0 2个体的外周血单个核细胞 (PBMC) ,与流感病毒特异的HLA A 0 2 0 1限制性CTL表位多肽 (IMP5 8 6 6 ,GILGFVFTL)孵育 ,ICCS检测CD8+ T细胞分泌细胞因子IFN γ、IL 2和TNF α。结果 :发现HLA A 0 2不同亚型的人PBMC对流感病毒多肽均具有记忆性免疫应答。结论 :①ICCS方法可特异性定量检测T细胞活化状态 ;②HLA A 0 2人群中普遍存在对IMP5 8 6 6多肽的记忆性免疫应答 ;③对于HLA A 0 2 0 1限制性多肽 ,与其它不同HLA A 0 2亚型间存在交叉反应性。Objective:Intracellular cytokine staining(ICCS) is applied to the detection of the immune response of peripheral blood mononuclear cells (PBMC)from subjects of different HLA A 02 supertype to influenza A matrix peptide.Methods:HLA A 02 subtype were identified by the way of SSP methods;PBMC from subjects with HLA A 02 were cocultured with influenza A matrix peptide (IMP) which was HLA A*0201 restricted CTL epitope, secreting cytokine such as IL 2, IFN γ,TNF α were measured by ICCS.Results:PBMC from HLA A 02 positive subjects had memory immune response to IMP.Conclusion:①The method of ICCS can be used for quantitive detecting T cell activating status specificly;②the memory immune response to IMP58 66 peptide exists in all of the HLA A 02 positive persons;③among different HLA A 02 subtype, there are cross reactivity to HLA A 0201restricted peptide.

关 键 词：细胞因子 免疫应答 HLA-A^＊02 CD81·T细胞 

分 类 号：R392.2[医药卫生—免疫学]