山茱萸总甙滴眼液防治角膜移植免疫排斥反应的实验研究  

Effect of cornus officinalis glycosides ophthalmic solution on the corneal allograft rejection

作　　者：孟晶[1] 陈建苏[1] 李辰[1] 赵松滨[1] 杨皓庄[2] 张穗梅[2] 狄静芳[3] 

机构地区：[1]暨南大学医学院眼科,广东广州510632 [2]暨南大学病理生理学教研室,广东广州510632 [3]暨南大学组织移植与免疫中心,广东广州510632

出　　处：《中国病理生理杂志》2003年第4期511-513,T004,共4页Chinese Journal of Pathophysiology

基　　金：广东省卫生厅医学科学技术研究基金资助项目 (A2 0 0 2 32 2 );广东省中医药管理局科研课题 ( 30 2 0 0 4)

摘　　要：目的 :观察山茱萸总甙 (COG)滴眼液局部应用对角膜移植免疫排斥反应的影响。方法 :建立封闭群大鼠角膜移植模型 ,用裂隙灯显微镜记录及比较各组角膜排斥反应发生时间 ;并进行病理学和扫描电镜检查。结果 :病理学检查B、C组 15d时的角膜植片淋巴细胞浸润及新生血管明显少于A组。免疫组化显示 :ICAM - 1的表达在 15d时B、C组明显少于A组。结论 :COG滴眼液能有效地防治角膜移植免疫排斥反应。AIM: To observe the effect of cornus of ficinalis glycosides(COG) ophthalmic solution on the corneal allograft rejection by topical instillation. METHODS: The corneal transplantation model on the closed colony rats was established. The rejection time of all animals was recorded and compared by slit-lamp microscope. The pathologic changes were measured by immunohistochemistry and scanning electron microscope.RESULTS: The histopathological and immunohistochemistry findings showed that the lymphocytes, neovascularity and the expression of ICAM-1 in COG-treated group were significantly fewer than that in control group at 15 d after operation.CONCLUSION: COG ophthalmic solution prevents and suppresses the corneal allograft rejection.

关 键 词：山茱萸总甙 滴眼液 防治 角膜移植 免疫排斥反应 实验研究 

分 类 号：R285.5[医药卫生—中药学] R779.65[医药卫生—中医学]