胺碘酮、美托洛尔对家兔急性心肌梗死血小板活化、纤溶活性、内皮血管活性物质的影响  

Effect of amiodarone and metoprolol on platelet activation, fibrinolytic activity and vasoactive mediators in acute myocardial infarction in rabbits

作　　者：刘恒亮[1] 康运凯[2] 吴莉华[1] 开芸 郝冬琴[1] 柴建文[1] 

机构地区：[1]郑州市第五人民医院心内科,河南郑州450003 [2]郑州市第五人民医院检验科,河南郑州450003

出　　处：《中国病理生理杂志》2003年第4期518-521,共4页Chinese Journal of Pathophysiology

基　　金：郑州市科技局自然科学基金资助项目 (No .2 0 0 0 12 0 5 )

摘　　要：目的 :探讨胺碘酮、美托洛尔对兔急性心肌梗死 (AMI)血小板活化、纤溶活性、内皮血管活性物质的影响。方法 :新西兰家兔 5 0只 ,随机分为 5组 ,每组 10只。Ⅰ组 :假手术组 ,Ⅱ组 :AMI组 ,Ⅲ组 :AMI+利多卡因组 ,Ⅳ组 :AMI+胺碘酮组 ,Ⅴ组 :AMI+美托洛尔组 ;Ⅱ、Ⅲ、Ⅳ、Ⅴ组分别结扎冠状动脉左室支中点后 ,4h取血分别测定血栓素B2 (TXB2 )、6 -酮 -前列腺素F1α( 6 -Keto-PGF1α)、内皮素 (ET)、一氧化氮 (NO)和组织型纤溶酶原激活剂 (t-PA)、纤溶酶原激活剂抑制物 (PAI)水平 ;摘取心脏 ,测定心肌梗死范围。结果 :Ⅱ、Ⅲ、Ⅳ、Ⅴ组血浆TXB2 、ET、NO浓度和PAI活性显著高于Ⅰ组 (P <0 0 1) ,6 -Keto-PGF1α浓度和t-PA活性显著低于Ⅰ组 (P <0 0 1) ,Ⅱ、Ⅲ、Ⅳ组之间比较 ,血浆TXB2 、6 -Keto-PGF1α浓度、t-PA活性及心肌梗塞范围无显著差异 (P >0 0 5 ) ,Ⅳ组血浆ET、NO浓度和PAI水平明显低于Ⅱ组 (P <0 0 1) ,Ⅴ组血浆TXB2 、ET、NO浓度和PAI水平明显低于Ⅱ组 (P <0 0 1) ,6 -Keto -PGF1α浓度、t-PA活性显著高于Ⅱ组 (P <0 0 1) ,心肌梗塞范围小于Ⅱ组 (P <0 0 1)。结论 :胺碘酮抑制AMI早期PAI活性 ,减少ET和NO的释放 ;美托洛尔抑制AMI早期血小板活化 ,改善纤溶活性 ,减少ET和NO的再释放 ,缩小?AIM: To explore the significance of platelet activation, fibrinolytic activity and the changes of vasoactive mediators in acute myocardial infarction in rabbits and the intervention of amiodarone and metoprolol. METHODS: Fifty New Zealand white rabbits were randomly assigned to five groups, ten for each. Group Ⅰ: sham group, group Ⅱ: acute myocardial infarction(AMI) group, group Ⅲ: AMI and lidocaine group, group Ⅳ: AMI and amiodarone group, group Ⅴ: AMI and metoprolol group.The middle point of left ventricular coronary artery was ligated (groupⅡ,Ⅲ, Ⅳ and Ⅴ ) or a sham ligation(group Ⅰ). Four hours later, blood was collected for measuring plasma concentration of TXB 2, 6-Keto-PGF 1α, ET, NO, plasma activity of t-Pa and PAI.After that, the heart was taken out to evaluate the infarction size(IS). RESULTS: Plasma concentration of TXB 2, ET, NO and plasma activity of PAI were significantly higher in groupⅡ,Ⅲ, Ⅳ and Ⅴ than those in group Ⅰ(P<0.01), but the plasma concentration of 6-Keto-PGF 1α and plasma activity of t-Pa were remarkably lower in groupⅡ,Ⅲ, Ⅳ and Ⅴ than those in group Ⅰ(P<0.01). There were no difference in plasma concentration of TXB 2, 6-Keto-PGF 1α, t-Pa activity and infarction size in group Ⅱ,Ⅲ and Ⅳ(P>0.05).Compared to group Ⅱ, plasma concentration of ET, NO and PAI activity were significantly decresed (P<0.01)in group Ⅳ. Plasma concentration of TXB 2, ET, NO and plasma activity of PAI were significantly lower in groupⅤ than those in group Ⅱ(P<0.01). Conversely, plasma concentration of 6-Keto-PGF1 and plasma activity of t-Pa were remarkably higher in group Ⅴ than those in group Ⅱ(P<0.01). The infarction size was remarkly decrease(P<0.01)in group Ⅴ. CONCLUSIONS: Amiodarone inhibited PAI avtivity, decreased release of ET and NO in AMI in rabbits. Metoprolol inhibited platelet activation, improved fibrinolytic, decreased release of ET and NO, and reduced myocardial infarction size in AMI in rabbits; Lid

关 键 词：胺碘酮 美托洛尔 家免 急性心肌梗死 血小板活化 纤溶活性 一氧化氮 

分 类 号：R542.22[医药卫生—心血管疾病]