卡介苗对豚鼠实验性哮喘的预防性治疗作用  

Effect of BCG on experimental asthma in a guinea pigmodel of allergen sensitization

作　　者：刘金保[1] 钟南山[2] 李树浓[3] 梁仲培[1] 王桂房[1] 吴卫[1] 

机构地区：[1]广州医学院病理生理学教研室,广东广州510182 [2]广州医学院呼吸疾病研究所,广东广州510182 [3]中山大学中山医学院病理生理学教研室,广东广州510089

出　　处：《中国病理生理杂志》2003年第4期464-467,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .3980 0 0 5 9) ;广东省自然科学基金资助项目 (No.980 46 0 )

摘　　要：目的 :观察卡介苗 (BCG)对哮喘豚鼠的预防治疗作用。方法 :采用 31只豚鼠 ,分为 3组进行处理 ,分别为对照组、卵蛋白 (OVA)致敏组和BCG处理组。用OVA(Ⅲ级 )致敏豚鼠复制豚鼠哮喘模型。结果 :本模型采用10 %的OVA致敏 ,1%的OVA激发 ,所有动物都表现有不同程度的过敏反应症状。实验动物在接受BCG注射后 ,表现为以下特点 :一是外周血淋巴细胞和单核细胞增加 ;二是BALF中细胞分类的变化 ,支气管肺泡灌洗液 (BALF)中以淋巴细胞的增加最为明显。经过OVA致敏的动物BALF和肺组织中嗜酸性粒细胞 (EOS)明显增加 ,BCG不同程度地降低肺组织EOS的气道浸润及减轻OVA致敏豚鼠的气道反应。结论 :使用本实验体系BCG可以减轻实验性哮喘的气道炎症反应。AIM: To examine the effect of bacillus calmette-guerin (BCG) on experimental asthma in guinea pigs. METHODS: Guinea pigs were sensitized with BCG and then with ovalbumin (ip). Two weeks later, guinea pigs were challenged with ovalbumin (OVA) aerosol inhalation. Thirty one Guinea pigs were divided into three groups at random control group,OVA-treated group, BCG and OVA-treated group.RESULTS: Ovalbumin inhalation caused a marked airway infiltration of eosinophils and all the animals exhibit asthmatic symptoms. Pretreatment with BCG induced typical increase in lymphocytes and monocytes in peripheral blood and in bronchoalveolar lavage fluid (BALF). BCG markedly inhibited eosinophil infiltration and attenuated the asthmatic symptoms. CONCLUSION: These data suggest that BCG exerts an inhibitory effect on asthmatic inflammation.

关 键 词：卡介苗 豚鼠 实验性哮喘 预防性治疗 

分 类 号：R562.25[医药卫生—呼吸系统] R186.2[医药卫生—内科学]