草问荆总生物碱对大鼠脑内氨基酸类神经递质含量和纹状体内乙酰胆碱含量的影响  

Effect of total alkaloids of Equisetum pratense on amino acid neurotransmitters and Ach of striatum in rat brain

作　　者：季宇彬[1] 高世勇[2] 

机构地区：[1]哈尔滨商业大学药物研究所博士后科研工作站,黑龙江哈尔滨150076 [2]哈尔滨商业大学制药工程系,黑龙江哈尔滨150076

出　　处：《中草药》2003年第4期338-340,共3页Chinese Traditional and Herbal Drugs

基　　金：黑龙江省科委自然科学基金资助项目 ( D0 1-0 5 )

摘　　要：目的　研究草问荆总生物碱 ( TAEP)对大鼠脑内氨基酸类神经递质含量和纹状体内乙酰胆碱 ( Ach)含量的影响 ,揭示其对中枢神经系统抑制作用的作用机制。方法　采用双波长扫描定量法和豚鼠回肠生物测定法观察大鼠脑内氨基酸类神经递质的含量和纹状体内 Ach含量。结果　 TAEP对大鼠脑内 4种氨基酸类神经递质 (谷氨酸、甘氨酸、γ-氨基丁酸、天冬氨酸 )的含量均无影响 ,但可显著降低大鼠纹状体内 Ach的含量。结论　 TAEP对中枢神经系统的抑制作用与脑内 4种氨基酸类神经递质 (谷氨酸、甘氨酸、γ-氨基丁酸、天冬氨酸 )的含量无关 ,而是通过降低 Ach的含量 ,进而影响多巴胺 - 2 ( DA- 2 )受体达到的。Object To study the effect of total alkaloids of Equisetum pratense Ehrh. (TAEP) on the contents of amino acid neurotransmitters and Ach in rat brain to reveal the mechanism of TAEP inhibitory action on the central nervous system (CNS). Methods Contents of amino acid neurotransmitters and Ach in rat brain were determined by double-wavelengh scan and GPI mensuration. Results TAEP could not influence four kinds of content of amino acid neurotransmitter (glutamic acid, glycin, γ-aminobutyric acid, aspartic acid), but TAEP could significantly lower the content of Ach in striatum of rat. Conclusion The inhibition of TAEP to CNS is attained by lowering the content of Ach in striatum to affect DA-2 receptor, and it is irrelevant to the amino acid neurotransmitters (glutamic acid, glycin, γ-aminobutyric acid, aspartic acid).

关 键 词：草问荆总生物碱 大鼠 脑内氨基酸类神经递质 含量 纹状体内乙酰胆碱 

分 类 号：R285.5[医药卫生—中药学]