滋阴活血润肠法与通里攻下法对失血性休克大鼠T淋巴细胞数量变化影响的对比研究  

Contrast Investigation of the Effect of the Treatment of Nourishing Yin and Promoting Blood Circulation to Lubricate Intestines and Dispelling Interior Pathogenic Factors and Puragation on changes in T Lymphocytes in Rats Subjected to Hemorr

作　　者：王银山[1] 蔡光先[1] 陈立峰[1] 喻正科[1] 周兵[1] 

机构地区：[1]湖南省中医药研究院,湖南长沙410006

出　　处：《中医药学刊》2003年第4期521-523,共3页Study Journal of Traditional Chinese Medicine

基　　金：湖南省自然科学基金资助项目(01JJY2041)

摘　　要：目的 :探讨滋阴活血润肠法与通里攻下法对伤后免疫功能的影响及对肠黏膜屏障的保护作用。方法 :70只SD大鼠随机分为休克模型组 (A组 )、空白对照组 (B组 )、滋阴活血润肠组 (C组 )和通里攻下组 (D组 ) ,以右侧颈动脉插管放血制作休克模型 ,空白对照组插管不放血 ,治疗组在伤前 3小时及伤后 6小时、12小时分别给予滋阴活血润肠中药 (便可通 )和大承气汤灌胃 ,休克模型组和空白对照组用生理盐水灌胃 ,伤后 2 4小时无菌条件下用下腔静脉取血处死 ,检测相应指标。结果 :C组CD3+ 、CD4 + 数量高于D组 ,差异有显著性 (P <0 .0 5 )。各组CD8+ 变化不大。C组CD4 + /CD8+ 值有 4 5 %在正常范围 ,D组只有 2 0 %在正常范围 ,差异有显著性 (P <0 .0 5 )。结论 :滋阴活血润肠法与通里攻下法均具有正相免疫调节功能 ,滋阴活血润肠法的作用优于通里攻下法 ,二者均具有明显肠黏膜屏障保护作用。Objective:To study the effect of the treatment of Nourishing Yin and Promoting Blood Circulation to lubricate intestines(NPL)and Dispelling Interior Pathogenic Factors and Purgation(DP)on immune function after wound and protective effects on intestinal mucous membrane barrier.Method:70 SD rats were divided randomly into shock model group,blank-controlled group,NPL group and DP group.And letting blood by arterial cannulation was used in shock model group but arterial cannulation without letting blood was used in blank-controlled group.And rats in treated group were given the traditional Chinese medicine herb(Bian Ke Tong),which has the action of NPL,and Da Cheng Qi Tang spearately,by perfusion of stomach 3 hours before operation and 6 hours?12hours after operation.Rats in shock model group and blank-controlled group were given saline by perfusion of stomach.All the rats were killed by drawing blood through inferior vena cava under the aseptic condition.Then corresponding markers were detected.Result:The quantities of CD3 + and CD4 + in NPL group were much more than ones in DP group and the difference is obvious(P<0.05).But the quantities of CD8 + in each group had no change.45% of the numeric values of CD4 +/CD8 +in NPL group belong to normal range;Only 20% of the ones in DP group belong to normal range,the difference between them is obvious (P<0.05).Conclusion:The treatment of NPL and DP both have the positive immune function and the former is better than the latter.They both have obviously proteefive effects on intestinal mucous membrane barrier. 〔

关 键 词：滋阴活血润肠法 通里攻下法 T淋巴细胞 肠黏膜屏障 免疫功能 

分 类 号：R241.2[医药卫生—中医诊断学]