PGE_2及cAMP在烧伤合并内毒素血症骨髓粒单系造血变化中作用的实验研究  

An experimental study on the role of PGE_2 and cAMP on the postburn change in the granulopoiesis in bone marrow in burned mice with endotoxemia

作　　者：向德兵[1,2] 刘友生[1] 王水明[1] 王晓东[1] 

机构地区：[1]第三军医大学西南医院病理研究所 [2]第三军医大学大坪医院病理科,400042

出　　处：《中华烧伤杂志》2003年第2期78-81,共4页Chinese Journal of Burns

基　　金：国家自然科学基金资助项目 ( 3 9770 75 9)

摘　　要：目的　探讨前列腺素E2 (PGE2 )、环磷酸腺苷 (cAMP)在烧伤合并内毒素血症骨髓粒单系造血变化中的作用。　方法　 178只昆明种小鼠随机分为烧伤合并内毒素注射组、单纯烧伤组、单纯内毒素注射组和等渗盐水对照组。应用放射免疫分析和免疫组化法 ,动态检测各组小鼠致伤后 1周内 ,骨髓细胞环氧酶 2 (COX 2 )表达及PGE2 、cAMP含量 ,并对粒单系造血变化进行了动态观察。　结果　烧伤合并内毒素血症早期骨髓粒单系略呈增生反应 ,继而呈现造血抑制。骨髓细胞COX 2表达、骨髓细胞上清PGE2 和细胞内cAMP在 12h～ 5d显著升高 ,且cAMP与PGE2 呈显著正相关 (r=0 .978,P <0 .0 1) ,而与粒 巨噬细胞集落形成单位 (CFU GM)呈显著负相关 (r =- 0 971,P <0 .0 1)。　结论　PGE2 在烧伤合并内毒素血症骨髓粒单系抑制中起重要作用 ,抑制效应是通过其与特异性受体结合 ,激活腺苷酸环化酶 。Objective To investigate the role of PGE 2 and cAMP in the postburn change in granulopoiesis in bone marrow in burned mice with endotoxemia. Methods One hundred and seventy eight mice were randomly divided into burn with LPS administration, simple burn, simple LPS administration and control (injection of normal saline) groups. The COX 2 expression and the contents of PGE 2 and cAMP in myeloid cells in injured mice in all groups were determined by RIA (radioimmuno assay) within 1 postburn week and immunohistochemistry methods. At the same time the change in granulopoiesis was dynamically observed. Results The granulopoiesis was enhanced slightly at the early stage of burn and with endotoxin challenge,followed by suppression. The COX 2 expression in myeloid cells the contents of PGE 2 on supernatant of marrow cells and intracellular cAMP in the myeloid cells was increased at 12 postburn hour(PBH) up to 5 postburn day (PBD). Furthermore, the change in the cAMP was evidently and positively correlated with that of PGE 2 (r=0.978, P<0.01), but was negatively correlated with that of CFU GM (r=-0.971,P<0.01) Conclusion PGE 2 might play pivotal roles in the postburn granulopoiesis suppression in bone marrow during endotoxemia. This effect might be accomplished by its ligating to its special receptor and to activate adenylate cyclase so as to increase the intracellular content of cAMP in bone marrows.

关 键 词：前列腺素E2 环磷酸腺苷 烧伤 内毒素血症 髓粒单系造血 

分 类 号：R644[医药卫生—外科学] R631.1[医药卫生—临床医学]