Ti质粒对甘薯叶片和愈伤组织的转化  

In Vitro Transformation of Leaves and Calli from Sweet Potato by Ti-Plasmid

作　　者：朱宝成[1] 王俊刚[1] 李庆余 

机构地区：[1]河北大学生物系

出　　处：《河北大学学报（自然科学版）》1992年第1期55-58,共4页Journal of Hebei University(Natural Science Edition)

摘　　要：用根癌农杆菌菌株T_(37)和B_6S_3感染甘薯叶片和愈伤组织,在无激素的MS培养基上诱发产生了转化愈伤组织。两菌株对愈伤组织的转化率明显高于对叶片的转化率。电泳结果表明,转化组织中均具有LpDH或NpDH活性,证明Ti质粒上的T-DNA已转移到甘薯细胞的基因组中。同时还进行了转化组织的过氧化物同工酶分析。The leaf explants and calli of sweet potato were cocultivated with the Agrobacterium tumefaciens. The transferred calli were induced on the phytohormone free MS medium in 2-3 weeks. The higher frequencies were got from the calli. There were NpDH or LpDH activity in transferred tissue, but there were no that activity in non-transferred tissue. It was showed that the leaves and calli of sweet potato were transferred by the bacterium. The peroxidase isozymes were contrasted in transferred and non-transferred tissue at the same time.

关 键 词：甘薯 根癌农杆菌 钛质粒 愈伤组织 

分 类 号：S531[农业科学—作物学]