固相萃取-高效液相色谱法同时测定克伦特罗和沙丁胺醇  

Simultaneous Analysis of Clenbuterol and Salbutamol by High Performance Liquid Chromatography/Solid Phase Extraction

作　　者：尚红霞[1] 卢业玉[1] 黄宝华[1] 何欣[1] 

机构地区：[1]广东工业大学轻化学院,广州510090

出　　处：《分析科学学报》2003年第2期145-147,共3页Journal of Analytical Science

摘　　要：提出用固相萃取 -高效液相色谱法同时测定饲料中微量克伦特罗和沙丁胺醇的新方法。选用 Dikma Diamonsil C18-ODS分析柱 (2 0 0× 4.6mm,5μm) ,乙腈 -0 .0 1mol/ L KH2 PO4 (p H 3 .0 )作流动相 ,应用波长编程进行检测。克伦特罗和沙丁胺醇的线性范围为 0 .1～ 1 0 0μg/ m L,相关系数分别为 0 .99999和 0 .99977,检出限分别为0 .3 1 ng/ m L和 0 .2 4ng/ m L ,回收率分别为 91 .2 %～ 92 .0 %和 91 .9%～ 93 .0 % ,相对标准偏差分别是 1 .2 0 %～ 2 .0 5%和 1 .2 9%～ 2 .51 %。A procedure for the simultaneous determination of clenbuterol and salbutamol in feed samples by high performance liquid chromatography/solid phase extraction has been proposed. The analysis was carried out with Diamonsil C 18 column, acetonitrile/water (0.01 mol/L KH 2PO 4,pH3.0)as mobile phase and wavelength program. The range of linear relationship of clenbuterol and salbutamol was 0.1～100 μg/mL ( r =0.99999 and 0.99977). The minimum detectable limit was 0.31 ng/mL and 0.24 ng/mL, respectively. The standard recovery was 91.2%～92.0% and 91.9%～93 0%, respectively. RSD was 1.20%～2.30% and 1.29～2.58%, respectively.

关 键 词：固相萃取 高效液相色谱法 同时测定 克伦特罗 沙丁胺醇 饲料添加剂 合成饲料 分析 

分 类 号：S816.2[农业科学—饲料科学] S816.7[农业科学—畜牧学]