SZ-51检测内皮细胞P-selectin表达及其对内皮与粒细胞粘附的影响  

Use of SZ-51 to Detect P-selectin Expressed on Endothelial cell and the Influence of SZ-51 on Polymorphonuclear Granulocyte Adhesion to Endothelial cell

作　　者：王晓岚[1] 赵益明[1] 何杨[1] 沈文红[1] 阮长耿[1] 

机构地区：[1]苏州大学附属第一医院江苏省血液研究所血检室,苏州215006

出　　处：《苏州大学学报（医学版）》2003年第2期132-134,共3页Suzhou University Journal of Medical Science

基　　金：国家攀登专项计划基金资助课题 (95 -专 -10 )

摘　　要：目的　观察单克隆抗体SZ - 5 1对内皮细胞P -selectin表达的检测效果及其对中性粒细胞与血管内皮细胞粘附的影响作用。方法　用流式细胞术检测活化内皮细胞P -selectin表达 ;用虎红染色法检测SZ - 5 1对中性粒细胞与血管内皮细胞粘附的影响。结果　SZ - 5 1能有效检出活化内皮细胞P -selectin表达 ,但对中性粒细胞与P -selectin、中性粒细胞与脂多糖活化的内皮细胞粘附无影响作用。结论　SZ - 5 1不是PObjective To observe the effect of SZ-51 for measurement of P-selectin Expressed on Endothelial cell and the influence of SZ-51 on polymorphonuclear granulocyte (PMN) adhesion to vascular endothelial cell. Methods P-selectin Expressed on Endothelial cell were detected by flow,cytometry (FCM).The influence of SZ-51 on PMN adhesion to P-selectin or to endothelial cell was observed by staining the cells with Rose Bengal.Results SZ-51 could detect the antigen expressed on the surface of endothelial cell in activated state.Treating P-selectin or endothelial cell with SZ-51 had no influence on adhesion of PMN to P-selectin or to HUVEC activated by lipopholysaccharide (LPS).Conclusion SZ-51 is not an inhibitive antibody against P-selectin.

关 键 词：SZ-5l 检测 内皮细胞 P-SELECTIN 表达 流式细胞术 虎红染色法 单克隆抗体 

分 类 号：R392－33[医药卫生—免疫学]