甲状腺过氧化物酶抗体检测方法的建立及临床应用  

Establishment of an RIA of serum thyroid peroxidase antibody and evaluation of its clinical use

作　　者：邵新华[1] 刘树业[1] 张卫民[1] 李涛[1] 周津东[2] 

机构地区：[1]天津市第三中心医院检验科,300170 [2]天津医科大学总医院内分泌科

出　　处：《中华核医学杂志》2003年第2期90-91,共2页Chinese Journal of Nuclear Medicine

基　　金：天津市卫生局科研基金资助项目 (960 0 5)

摘　　要：目的　建立血清甲状腺过氧化物酶抗体放射免疫检测方法 ,探讨其对甲状腺疾病的临床意义。方法　采用1 2 5I氯胺 T法标记人甲状腺过氧化物酶 ,选择不同的实验条件 ,进而确定实验方法。测定 37例正常对照者和 92例甲状腺疾病患者血清甲状腺过氧化物酶抗体。结果　甲状腺疾病患者血清甲状腺过氧化物酶抗体水平显著高于对照组 (F =4 .6 12 ,P <0 .0 5 ) ,各种甲状腺疾病的阳性检出率分别为 :Graves病 6 6 .7% ,桥本甲状腺炎 95 .2 % ,原发性粘液水肿 72 .7%。结论　该方法特异性强 ,重复性好 ,非特异性结合率低 。Objective RIA of serum thyroid peroxidase (hTPO) antibody was established and its clinical significance was evaluated by measuring levels of serum hTPO antibody. Methods hTPO was labelled by 125 I and the best conditions were selected. The levels of serum hTPO antibody were measured in 37 normal controls and 92 patients with autoimmune thyroid diseases (AITD). Results Analysis of variables showed that the level of serum hTPO antibody was significant higher in patients with AITD than that in normal controls ( F =4.612, P <0.05). Positive rates of serum hTPO antibody in Graves' disease,Hashimoto's thyroiditis and primary myxedema were 66.7%, 95.2% and 72.7%, respectively. Conclusion This method is of high specificity and sensitivity, good reproducibility, low in nonspecfic binding and useful to study and diagnose AITD.

关 键 词：甲状腺疾病 过氧化物酶 抗体 检测方法 建立 临床应用 放射免疫检测 

分 类 号：R817.4[医药卫生—影像医学与核医学]