多囊卵巢综合征患者中未成熟卵母细胞体外培养成熟后体外受精的初步研究  

The primary research on the technique of in vitro maturation of immature oocyte and in vitro fertilization in PCOs patients.

作　　者：李媛[1] 陈子江[1] 赵力新[1] 李梅[1] 唐蓉[1] 许成岩[1] 马水英[1] 

机构地区：[1]山东大学临床学院山东省立医院生殖医学中心,济南250021

出　　处：《现代妇产科进展》2003年第2期111-113,共3页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨未成熟卵母细胞体外培养成熟后体外受精、胚胎培养技术在多囊卵巢综合征患者中的初步应用及其影响因素。方法:用小剂量促性腺激素促使卵泡生长后,根据优势卵泡直径分为2组,直径6～8mm者为组1,10—12mm者为组2。采集未成熟卵母细胞,经体外培养成熟后,再进行体外授精和胚胎培养。结果:组1的GV期卵的成熟率和受精率低于组2者,但两组的MI期卵的成熟率、受精率没有明显差别。两组卵裂率没有明显差别,但形成胚胎的质量组2优于组1。总计成熟率69.3％,成熟卵中正常受精率61.5％。结论:可以用小荆量Gn促使卵泡生长后,采集未成熟卵,用体外成熟-体外授精(IVM/IVF)的方法使多囊卵巢患者在避免OHSS的情况下获得质量良好的胚胎。Objective:To study the application of the technique of in vitro maturation,fertilization and embryo culture of immature oocyte in PCOs patients and some factors affecting the process . Methods: The immature oocyte were retrieved priming with little amount of FSH. According to the size of the follicles, there were 2 groups. Group 1 were the oocytes from the 6 ~ 8mm follicles, and group 2 from 10 - 12mm. After in vitro maturation (IVM) and fertilized, the embryos were scored. Results: The maturation and fertilization rates of the GV stage oocytes from group 1 were lower than those from group 2, but there was no difference in the maturation rate and fertilization rate of the MI stage oocytes between the two groups. There was also no difference in the cleavage rate, but the quality of the embryos from group 2 was higher than that from group 1. In total, the maturation rate was 69.3%,normal fertilization rate was 61.5% among the matured oocytes.Conclusions:It may be useful to picking up the immature oocytes priming with little amount of gonadotropin to improve the development of small follicles, and then proceeding IVM/IVF. Using this technique can let the PCOs women achieve embryos of good quality without OHSS.

关 键 词：卵母细胞 细胞 体外受精 胚胎培养 体外培养 多囊卵巢综合征 

分 类 号：R711.6[医药卫生—妇产科学]