HPLC测定牛黄解毒片中大黄素、大黄酸的含量  

Determination of emodin and rhein in Niuhuangjiedu Tablets by HPLC

作　　者：李太平 王小文 刘东权 

机构地区：[1]丹东市药品检验所,辽宁丹东118002 [2]丹东锦江制药厂,辽宁丹东118000

出　　处：《中成药》2003年第4期293-295,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :建立一种简便的测定牛黄解毒片 (牛黄、雄黄、石膏、大黄、黄芩、桔梗、冰片、甘草 )中大黄素、大黄酸的含量测定方法。方法 :色谱柱为Diamonisil(TM 钻石 )C18柱 (2 0cm× 4 .6mm ,5 μm) ,流动相为甲醇 水 磷酸 (72 0∶2 80∶1) :检测波长2 5 4nm ;流速 1.5mL·min-1。大黄素保留时间为 15∶5 6min ,大黄酸保留时间为 8.4 9min。结果 :大黄素进样量在 0 .4 6 9～1.4 0 7μg时呈良好的线性关系 (r =0 .9992 ) ;大黄酸在进样量为 0 .312 5～0 .9375 μg时呈良好的线性关系 (r =0 .9992 ) ;加样回收率分别为 98.2 % (大黄素 ) ,98.4 % (大黄酸 ) ,RSD分别为 0 .4 3% (大黄素 ) ,0 .72 % (大黄酸 )。结论 :本法操作简便 。Objective: A HPLC method for determination of emodin and rhein in Niuhuangjiedu Tablets was established. Methods: The chromatographic conditions were listed below: Diamonisil ODS C 18 column, UV detector at 254nm of wavelength, a mobile phase of methanol water phosphate acid(720∶280∶1),and 1mL·min -1 of flow rate. The retention time of emodin was 8.49 min and that of rhein was 15.56min. Results: The calibration curves were linear for emodin within 0.469～1.407μg( r =0.9992) and for rhein within 0.3125～ 0.9375μg ( r = 0.9992). The recoveries were 98.2%( RSD =0.43%)for emodin and 98.4%( RSD =0.72%)for rhein, respectively. Conclusion: This method is simple and the separate result is satisfactory.

关 键 词：大黄酸 大黄素 牛黄解毒片 HPLC 

分 类 号：R927.2[医药卫生—药学]