HIV感染对记忆型和处女型CD4^+T细胞上CD95的影响  

Effect of HIV-1 infection on apoptosis of CD4^+T lymphocytes mediated by Fas

作　　者：吴南屏[1] 李丹[1] Armin Bader Hoxtermann Stefan Norbert Brockmeyer 

机构地区：[1]浙江大学医学院附属第一医院传染病研究所,浙江杭州310003 [2]德国鲁尔大学约瑟夫艾滋病研究中心

出　　处：《浙江大学学报（医学版）》2003年第2期90-93,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：国家留学回国人员基金资助项目 (988330 5 7)

摘　　要：目的 :了解 CD95在 HIV感染者记忆型和处女型 CD4 + T细胞表面的表达情况 ,探讨 Fas/ CD95在 HIV感染中的作用。方法 :采用流式细胞检测仪 (FACS)对 HIV感染者外周血 CD4 + T细胞表面 CD95、CD4 5 RA、CD4 5 RO的表达进行分析 ,用 EL ISA检测血清中 Fas水平。结果 :HIV感染后 ,血清 Fas水平随疾病的进展而逐渐升高 ,同时伴有处女型 T细胞表面 CD95表达增高以及记忆型 T细胞 CD95表达逐渐下降。结论 :Fas参与了 HIV的感染过程。研究 Fas和 CD95、CD4 5 RA、CD4 5 RO之间的关系 ,可以加深对 HIV致病机制的认识 ,并可了解疾病的进展情况和指导治疗。Objective: To study the pathogenic role of Fas/CD95 in HIV 1 infection subjects, and to investigate the effects of HIV on plasma levels of sFas and the expression of CD95 on different CD4 + T lymphocyte subpopulations. Methods: Four color flow cytometry was used to determine the expression of CD95,CD45RO,CD45RA on CD4 + T lymphocyte in peripheral blood from HIV 1 infection subjects and serum Fas levels were quantified by enzyme linked immunosorbent assay (ELISA). Results: Compared with healthy controls, serum Fas levels were significantly increased ( P <0.05) in HIV group and positively correlated with the disease progress. The expression of CD95 on navie T lymphocyte subsets was increased whereas that on memory T lymphocyte subsets was decreased. Conclusion: Fas plays an important role in the deletion of CD4 + T lymphocyte during HIV 1 infection. Further understanding of the relationship between Fas/CD95 and CD45RO/CD45RA may help to predict the progression of the disease and the clinical outcome.[

关 键 词：HIV感染 记忆型 处女型 CD4^-T细胞 CD95 艾滋病 流式细胞检测 

分 类 号：R512.91[医药卫生—内科学]