HIV重叠肝炎病毒感染者血清IL-18和IL-10水平分析  

Evaluation of serum interleukin-18 and interleukin-10 in patients with HIV-1 and hepatitis viruses co-infected subjects

作　　者：吴南屏[1] 李丹[1] Armin Bader Hoxtermann Stefan Norbert Brockmeyer 

机构地区：[1]浙江大学医学院附属第一医院传染病研究所,浙江杭州310003 [2]德国鲁尔大学约瑟夫艾滋病研究中心

出　　处：《浙江大学学报（医学版）》2003年第2期94-96,共3页Journal of Zhejiang University(Medical Sciences)

基　　金：国家留学回国人员基金资助项目 (988330 5 7)

摘　　要：目的 :探讨细胞因子在 HIV- HBV/ HCV重叠感染中的意义。方法 :采用 ELISA法检测血清 IL- 18和 IL- 10的水平 ,荧光定量 PCR计数测定 HIV- RNA滴度 ,流式细胞仪检测 CD4 + T细胞。结果 :HIV感染后 ,血清 IL- 18显著高于正常对照 (P<0 .0 5 )。随着疾病的进展 ,IL- 18逐渐上升而 IL - 10逐渐下降。重叠感染组血清 IL - 18和 IL- 10显著高于单纯 HIV感染组。结论 :肝炎病毒感染是影响 HIV感染者血清细胞因子水平的一个协变量 ,上调 IL- 18和下调 IL - 10可能起到改善重叠感染的预后的作用。Objective: To study the significance of cytokines in patients with HIV and hepatitis viruses co infection. Methods: Serum levels of IL 18 and IL 10 were measured by enzyme linked immunosorbent assay (ELISA). HIV RNA levels were measured in EDTA plasma by quantitative reverse polymerase chain reaction (PCR). CD4 + lymphocyte counts were determined by four color Flow cytometery (FCM). Results: The levels of IL 18 were significantly higher in HIV infected persons compared with those in controls ( P <0.05). With HIV disease progression, IL 18 levels increased while Il 10 levels decreased. HCV patients showed lower levels of IL 18 and IL 10 than those of the co infection group. Conclusion: Univariate analyses shows significant co variables IL 10 in co infection . Up regulating IL 18 activity and/or down regulating IL 10 may be a potential therapy to patients with HIV and hepatitis viruses co infection.[

关 键 词：HIV感染 重叠感染 肝炎病毒感染 血清 IL-18 IL-10 

分 类 号：R446.6[医药卫生—诊断学]