内源性高甘油三酯血症患者血浆极低密度脂蛋白、低密度脂蛋白及高密度脂蛋白对血凝及纤维蛋白溶解的影响  

Effects of Plasma Very Low Density Lipoprotein,Low Density Lipoprotein and High Density Lipoprotein on Blood Coagulation and Fibrinolysis in Endogenous Hypertriglyceridemia

作　　者：邓祖跃[1] 刘秉文[1] 刘宇[1] 白怀[1] 张祖辉[1] 

机构地区：[1]四川大学华西基础医学与法医学院载脂蛋白研究室,四川省成都市610041

出　　处：《中国动脉硬化杂志》2003年第2期93-98,共6页Chinese Journal of Arteriosclerosis

基　　金：国家重点基础研究发展规划 973规划 (G2 0 0 0 0 5690 0 )资助

摘　　要：研究内源性高甘油三酯血症患者血浆极低密度脂蛋白、低密度脂蛋白及高密度脂蛋白是否发生了氧化修饰及其对凝血及纤维蛋白溶解活性的影响。对 2 1例内源性高甘油三酯血症患者与 2 1例年龄性别相近的正常人的血脂、脂质过氧化物进行了分析。用一次性密度梯度超速离心法分离血浆极低密度脂蛋白、低密度脂蛋白及高密度脂蛋白。测定这 3种脂蛋白的 2 34nm吸光度、相对电泳迁移率和硫代巴比妥酸反应物质含量。分别将这 3种脂蛋白加入由正常人新鲜混合血浆构成的反应系统中 ,按试剂盒分别测定凝血酶原时间、活化部分凝血酶原时间、组织型纤溶酶原激活物活性及纤溶酶原激活物抑制剂 1活性。内源性高甘油三酯血症患者血浆甘油三酯含量平均升高 2 .73倍 ,高密度脂蛋白胆固醇下降 1.71倍 ,同时硫代巴比妥酸反应物质含量升高 1.2 2倍 ;内源性高甘油三酯血症组极低密度脂蛋白、低密度脂蛋白及高密度脂蛋白的 2 34nm吸光度、相对电泳迁移率和硫代巴比妥酸反应物质含量均较对照组显著增加 (P <0 .0 1) ,表明内源性高甘油三酯血症患者血浆极低密度脂蛋白、低密度脂蛋白及高密度脂蛋白均发生了氧化修饰 ,生成了氧化极低密度脂蛋白、氧化低密度脂蛋白及氧化高密度脂蛋白。Aim To study whether plasma very low density lipoprotein (VLDL), low density Lipoprotein (LDL), high density lipoprotein (HDL) were oxidatively modified in endogenous hypertriglyceridemia and effects of VLDL, LDL and HDL on blood coagulation and fibrinolysis in vitro. Methods Plasma VLDL, LDL and HDL were isolated with density gradient ultracentrifugation method. Human plasma triglycerides (TG), total cholesterol (TC),high density lipoprotein cholesterol (HDLC) were measured by enzyme method. The oxidative modification of LDL, VLDL and HDL was identified by agarose gel relative electrophoretic mobility (REM), absorbance at 234 nm and fluorescence of thiobarbituric acid reaction substances (TBARS). Prothrombin time (PT),activated partial thrombplastin time (APTT), plasminogen activator inhibitor 1 (PAI 1) activity and tissue plasminogen activator (t PA) activity were measured in the reaction system consisted of freshly mixed normal plasma according to the direction of the kits. Results The hypertriglyceridemia (HTG) group were 1.6 and 0.4 times more plasma TG, TBARS level than the control group respectively (P<0.01). The plasma HDLC in HTG group was 32% lower than that of the control group (P<0.01). The rela tive electrophoretic mobility, absorbance at 234 nm and TBARS of VLDL, LDL and HDL in HTG group were significantly higher than that of the control group (P<0.01). The PT and APTT of VLDL, LDL and HDL in HTG group were significantly shorter than that of the control group (P<0.05). The t PA and VLDL in HTG group was higher and the PAI 1 in VLDL in HTG group was lower than that of the control group (P<0.01), but LDL and HDL were not influenced by the t PA and the PAI 1 in HTG group and the control group. The correlation analysis indicated that electrophoretic mobility of VLDL and HDL in HTG group was negatively correlated with PT (P<0.01). Conclusions Oxidative modification of plasma very low density lipoprotein, low density Lipoprotein, high density lipoprotein occurred in endogenous

关 键 词：内科学 脂蛋白与血凝及纤维蛋白溶解的关系 琼脂糖凝胶电泳 高甘油三酯血症 脂蛋白血症 纤维蛋白 血凝 纤维蛋白 

分 类 号：R589[医药卫生—内分泌]