葡萄糖、胰岛素和氧化低密度脂蛋白对内皮细胞内皮素-1生成及其mRNA水平的影响  

Effects of Glucose,Insulin and Oxidized LDL on Levels of ET-1and ET-1mRNA of Vascular Endothelial Cells

作　　者：柴伟栋[1] 陈家伟[1] 沈捷[1] 崔毓桂[1] 马立隽[1] 马向华[1] 倪黎[2] 汪承亚[2] 

机构地区：[1]南京医科大学第一附属医院内分泌科,江苏南京210029 [2]南京医科大学第一附属医院中心实验室,江苏南京210029

出　　处：《中国微循环》2003年第2期74-77,共4页Journal of Chinese Microcirculation

摘　　要：目的研究葡萄糖 (GLU)、胰岛素 (INS)和氧化低密度脂蛋白 (ox -LDL)对内皮细胞 (ECs)内皮素 -1(ET-1)分泌及其mRNA水平的影响 ,探讨ET-1分泌异常与DM血管并发症的关系。方法培养胎牛动脉内皮细胞 ,用放免法测定内皮细胞ET-1水平 ;半定量RT-PCR检测ET-1mRNA水平。结果(1)高浓度GLU、INS和ox-LDL促进ECs合成ET-1,并且这种作用有浓度和时间依赖性;高浓度GLU(20mmol/L～40mmol/L)并不增加ECsET-1mRNA的表达 ,但高浓度INS(1.8nmol/L～6.0nmol/L)和ox-LDL(25μg/ml～50μg/ml)能上调ET -1mRNA水平;(2)生理性低浓度INS0.18nmol/L下 ,ECs合成的ET-1及其mRNA水平均无明显增加 ;(3)高浓度GLU、INS、ox-LDL两两及三者间具有正性协同作用 ,促进ET-1的分泌 ;对ET-1的促分泌作用高浓度INS和ox-LDL可能要强于高血糖。结论高浓度GLU、INS和ox-LDL可直接导致血管ECs功能的异常 ,引起ET-1产生增多 ,促进DM血管并发症的发生、发展。Objective To explore the relationship between endothelin_1(ET_1)and diabetic vascular complications,the effects of various concentrations of glucose,insulin and ox_LDL on ET_1production and the levels of its mRNA of vascular endothelial cells in culture.Methods ET_1radioimmunoassay for ECs was used in this study,and the levels of ET_1mRNA were measured by semiquantiative RT_ PCR.Results(1)High concentrations of glucose(20mM～40mM),insulin(1.8nmol/L～6.0nmol/L)and ox_LDL(25μg/ml～50μg/ml)increased and caused concentration_and time_dependent ET_1release of ECs,and high concentrations of insulin and ox_LDLupregulated the level of ET_1mRNA.(2)Lowconcentration of insulin(0.18nmol/L)did not stimulate ET_1production and upregulate the level of ET_1mRNA of ECs.(3)High concentrations of glucose,inˉsulin and ox_LDL had synergetic effects on ET_1release,especially high insulin or oxidized LDL stimulated more ET_1release than high glucose.Conclusions High concentrations of glucose,insulin and ox_LDL can stimulate ET-1production,which leads to the dysfunction of vascular endothelial cells directly and accelerates the developˉment of diabetic vascular complications.

关 键 词：葡萄糖 胰岛素 氧化低密度脂蛋白 内皮细胞 内皮素-1 mRNA 糖尿病 血管并发症 

分 类 号：R587.2[医药卫生—内分泌]