卡托普利逆转压力负荷增加大鼠左室重构的机理研究  

Mechanism Research on Captopril Regressing Left Ventricular Remodeling In Rat with Pressure Overload

作　　者：蔡辉[1] 胡婉英[2] 王艳君[3] 郭郡浩[1] 胡兵[1] 姚茹冰[1] 

机构地区：[1]南京军区南京总医院中医科,江苏南京210002 [2]上海中医药大学附属曙光医院 [3]河北医科大学博士后流动站

出　　处：《中国微循环》2003年第2期89-92,共4页Journal of Chinese Microcirculation

摘　　要：目的观察卡托普利逆转压力负荷增加大鼠左室重构的作用及其机理。方法36只雄性Wistar大鼠随机分为假手术组、模型组、卡托普利组。建立腹主动脉狭窄所致充血性心力衰竭左室重构大鼠模型 ,观察左室质量指数 (LVMI) ,采用免疫组织化学染色方法观察心肌Ⅰ、Ⅲ型胶原的改变 ,采用RT-PCR方法观察心肌Ⅰ型和Ⅲ型胶原mRNA的表达 ,并采用放射免疫分析方法检测血浆和心肌血管紧张素Ⅱ (AngⅡ ) ,血浆心钠素 (ANF) ,血清醛固酮 (ALD)水平。结果模型组LVMI、Ⅰ型胶原、Ⅲ型胶原、Ⅰ型胶原mRNA表达明显高于假手术组 (P<0.001,P<0.01 ,P<0.05) ,卡托普利组LVMI、Ⅰ型胶原、Ⅲ型胶原、Ⅰ型胶原mRNA表达与模型组比明显下降 (P<0.05 ,) ;模型组Ⅲ型胶原mRNA与假手术组比较有显著升高(P<0.05) ,卡托普利组较模型组下降无显著差异(P>0.05)。模型组血浆AngⅡ、左室心肌AngⅡ、血浆ANF和血清醛固酮水平较假手术组显著升高 (P<0.001 ,P<0.01) ,卡托普利组明显降低血浆AngⅡ、左室心肌AngⅡ、血浆ANF和血清醛固酮水平 (P<0.01 ,P<0.001)。结论压力负荷增加大鼠心肌Ⅰ和Ⅲ型胶原及其mRNA的表达增加 ,具有左室重构的改变。卡托普利降低压力负荷增加大鼠心肌Ⅰ和Ⅲ型胶原及其mRNA的表达 ,具有逆转左室重构的作用。卡托普利逆转左?Objective To observe the effect of captopril regressing left ventricular remodeling in rats with pressure overload and to elucidate its possible mechanism.Methods36Wistar male rats were ranˉdomly divided into sham operation group,model group and captopril group.Observe left ventricular mass inˉdex(LVMI),cardiac tissue collagen typeⅠandⅢ,mRNA expression of collagen typeⅠandⅢ,plasˉma angiotensinⅡ(AngⅡ)and left ventricular tissue AngⅡ,plasma atrial natriuretic factor(ANF),serum aldosterone(ALD)level in rat with left ventricular remodeling of CHF by abdominal aortic constriction.Colˉlagen typeⅠandⅢwere measured by immunohistochemistry.mRNA expression of collagen typeⅠandⅢwas measured by RT-PCR.AngⅡ,ANF and ALD were measured by radioimmunoassay.Results LVˉMI,Collagen typeⅠ,Collagen typeⅢ,Expression of collagen typeⅠmRNA of model group were increased(P<0.001,P<0.01,P<0.05,),while those of captopril group were decreased(P<0.05).Expression of collagen typeⅢmRMA of the model group was increased(P<0.05,),while that of the captopril group was not decreased(P>0.05).The plasma and myocardial AngⅡ,plasma ANF, serum aldosterone of the model group were increased (P<0.001,P<0.01),while those of the captopril group were decreased(P<0.01and P<0.001).Conclusions Myocardial collagen typeⅠand typeⅢand the mRNA expression in rat with pressure overload are increased.There was change of regressing left ventricular remodeling.Myocardial collagen typeⅠand typeⅢand their mRNA expression in rat with pressure overload are decreased by captopril.There was effect of regressingleft ventricular remodeling.Captopril is effective in regressing myocardial fibrosis.The effect may be related to the decrease of circulating or local AngⅡlevel,plasma ANF,serum aldosterone and affectting expression of collagen typeⅠand typeⅢmRNA.

关 键 词：卡托普利 压力负荷 大鼠 左室重构 免疫组织化学染色 醛固酮 血清 血浆 心钠素 Ⅲ型胶原 Ⅰ型胶原 基因表达 充血性心力衰竭 主动脉狭窄 

分 类 号：R541.61[医药卫生—心血管疾病] R972[医药卫生—内科学]