NGF和BDNF在脊髓全横断后大鼠运动皮质的表达和分布  

The Expression and Distribution of NGF and BDNF in Adult Rat Motocortex Following Spinal Cord Complete Transection

作　　者：万祎[1] 陈熙[1] 何洁[2] 邓盘月[3] 罗学港[3] 

机构地区：[1]南华大学医学院解剖学教研室,湖南衡阳421001 [2]南华大学医学院病理学教研室 [3]中南大学湘雅医学院

出　　处：《南华大学学报（医学版）》2003年第1期1-4,54,共5页Journal of Nanhua University(Medical Edition)

基　　金：国家重点基础研究规划"脑功能和脑重大疾病的基础研究"(G1999054009)项目资助

摘　　要：目的 探讨脊髓全横断后神经营养因子在运动皮质内的分布规律,为临床应用神经生长因子治疗脊髓损伤提供实验依据。方法 用免疫组化方法和图像分析技术检测脊髓全横断后不同时相运动皮质内,神经生长因子(NGF)和脑源性神经营养因子(BDNF)的表达和分布。结果各组的运动皮质中NGF和BDNF阳性神经元主要分布在第V层;脊髓全横断后NGF和BDNF的表达均增高,NGF的表达高峰在脊髓全横断后7天,21天回到正常水平;BDNF表达在脊髓横断后21天到达高峰,28天回至正常水平。结论脊髓损伤后大鼠运动皮质内NGF表达上调早于BDNF。Objective To study the pattern of the distribution of NGF (nerve growth factor)and BDNF(brain - derived neurotrophic factor) in adult rat motocortex following complete spinal cord transection (SCT) .Methods With immunohistochemistly and quantitative analysis by PIPS - 2020 superhigh resolution pathology image analysis system, the expression and distribution of NGF and BDNF were investigated in motocortex following complete SCT. Results NGF and BDNF immunoreactive positive cells are mainly localized in motocortex layer V in both control and experimental groups. The expression of NGF in adult rat motocortex was up - regulated transiently after transection and reached the peak in day 7. The expression of BDNF was increased after SCT and reached the peak at day 21. Conclusion The up - regulation of NGF occurred earlier than that of BDNF in adult rat motocortex following SCT.

关 键 词：NGF BDNF 脊髓全横断 大鼠 运动皮质 分布规律 神经生长因子 脊髓损伤 神经营养因子 

分 类 号：R651.2[医药卫生—外科学]