反相高效液相色谱法测定咳欣康片中黄芩苷含量  

Content Determination of Baicaline in Kexinkang Tablets with Reversed Phase High Perfarmance Liquid Chromatography

作　　者：李锦绣[1] 高长清 杨丽华 贾善学[1] 

机构地区：[1]山东省滨州市人民医院药剂科,256610 [2]山东省莱芜市药品检验所,271100 [3]山东省滨州市药品检验所,256618

出　　处：《医药导报》2003年第5期330-331,共2页Herald of Medicine

摘　　要：目的 :采用反相高效液相色谱法 (RP HPLC)测定咳欣康片中黄芩苷的含量 ,以控制该制剂的质量。方法 :以C1 8化学键合硅胶柱分离黄芩苷 ,以甲醇 水 醋酸 ( 4 0∶60∶1)为流动相 ,检测波长 2 74nm进行测定。结果 :黄芩苷峰与其他组分峰的分离度为 5 .0 ,理论塔板数以黄芩苷峰计算为 2 5 870 ;平均加样回收率为 98.8% (n =5 ) ;5次独立测定的相对标准差为RSD =0 .92 %。黄芩苷在 0 .2 5～ 2 .5 0 μg范围内 ,进样量与吸收面积值呈良好的线性关系。 结论 :该法测定咳欣康片中黄芩苷的含量 ,结果准确 ,重复性好。Objective:To dominate the quality of kexinkang tablets by determining the content of baicaline in kexinkang tablets with reversed phase HPLC. Method:Baicaline was separated on spherisorb C 18 column by using methanol acetic acid water(40∶1∶60)as a mobile phase and UV detection wavelength at 274 nm. Results:The resolution was 5.0; The number of theoretical plates calculated for Paeoniflorin peak was 25 870; The average recovery of the added index was 98.8%( n =5). RSD got in the times of determination was 0.92%. There was a good linear relationship between the concentration of baicaline and absorption area value in the range of 0.25-2.50 μg. Conclusion:The methed was highly reproducible and accurate.

关 键 词：咳欣康片 黄芩苷 反相高效液相色谱法 含量测定 

分 类 号：R282.71[医药卫生—中药学] R927.2[医药卫生—中医学]