基于核壳荧光纳米颗粒的一种新型纳米pH传感器  

A Novel Nano-sensor Based on Fluorescent Core-shell Silica Nanoparticle for pH Measurements in Murine Macrophages

作　　者：段菁华[1] 王柯敏[1] 何晓晓[1] 谭蔚泓[1] 刘斌[1] 何春梅[1] 莫远尧[1] 黄杉生[1] 李杜[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室

出　　处：《湖南大学学报（自然科学版）》2003年第2期1-5,15,共6页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金 ( 2 0 13 5 0 10 ) ;国家杰出青年基金 ( 2 982 5 110 ) ;教育部重大项目基金 ( 2 0 0 0 -15 6);教育部骨干教师基金( 2 0 0 0 -65 )等资助项目

摘　　要：改进了一种基于新型的荧光染料——二氧化硅的核壳荧光纳米颗粒的纳米 p H传感器 ,以异硫氰酸荧光素 ( FITC)标记的羊抗人免疫球蛋白 Ig G为核材料 ,采用实验条件简单的油包水的微乳液方法制备荧光纳米颗粒 ,该方法有效地防止了荧光染料在二氧化硅壳层中的泄露 .这种 FITC的核壳荧光纳米颗粒对 p H敏感 ,在 p H值 5 .5～ 7之间呈线性响应 ,且能被单个小鼠巨噬细胞吞噬 ,借此用于单细胞中 pThe synthesis of fluorescent pH sensitive nanoparticles and the application of monitoring the intracellular pH in real time were described.A novel fluorescent core shell nanoparticle was developed by encapsulating the fluorescein isothiocyanate into silica shell with a reverse microemulsion technique.The FITC encapsulation is greatly enhanced conjuncting a water soluble protein IgG which significantly reduces FITC leaching from the silica matrix when immersed inside water.The hydrophilic silica shells are made by hydrolysing and polymerizing tetraethoxysilane.The fluorescent core shell nanoparticles are pH sensible and the pH dynamic range of the sensing nanoparticles is between 5.5 and 7.The nanoparticles can be phagocytosed by single murine macrophage to measure the change of intracellular pH value.They show the excellent stability and high reproducibility used as pH optical sensors.

关 键 词：油包水微乳液 核壳荧光纳米颗粒 PH传感器 小鼠巨噬细胞 

分 类 号：Q503[生物学—生物化学]