Study on Endophytic Bacteria Diversity in the Root of Peperomia dindygulensis Miq. by Applying Clone Library Construction Method  

作　　者：Song JIN Zebin CHEN Yuchuan LI Tiyuan XIA Zhen REN Lifang YIN 

机构地区：[1]Agriculture College,Kunming University [2]Key Laboratory of Characteristic Biological Resources Development and Utilization in Yunnan Province [3]Urban Characteristic Agriculture Engineering Technology Research Center of Yunnan Province

出　　处：《Medicinal Plant》2017年第6期13-16,共4页药用植物：英文版

基　　金：Supported by Scientific Research Foundation of Yunnan Provincial Department of Education(2015Y393);Scientific Research Project of Kunming University(XJZD1605);Construction Project of Key Superior and Characteristic Disciplines(Ecology)in Colleges and Universities of Yunnan Province(05000511311);Project of Key Laboratory of Special Biological Resource Development and Utilization of Universities in Yunnan Province(GXKJ201622)

摘　　要：[Objectives] To study the endophytic bacteria diversity in the root of Peperomia dindygulensis Miq. [Methods] The root of P.dindygulensis Miq. was taken as materials,culture-independent method was applied to build 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq. [Results] In the 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq.,95 clones separately belonged to 46 OTUs,Phylogenetic analysis indicated that these cloning sequences separately belonged to phylum Proteobacteria,phylum Bacteroidetes,phylum Actinobacteria,phylum Nitrospirae,and phylum Gemmatimonadetes. In these,phylum Proteobacteria accounted for 70. 53% of total number of clones,so it was the most dominant group in this library. [Conclusions]The endophytic bacteria diversity in the root of P.dindygulensis Miq. is rich and this study is expected to provide scientific basis for protection,development and use of resources of P. dindygulensis Miq.[Objectives] To study the endophytic bacteria diversity in the root of Peperomia dindygulensis Miq. [Methods] The root of P.dindygulensis Miq. was taken as materials,culture-independent method was applied to build 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq. [Results] In the 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq.,95 clones separately belonged to 46 OTUs,Phylogenetic analysis indicated that these cloning sequences separately belonged to phylum Proteobacteria,phylum Bacteroidetes,phylum Actinobacteria,phylum Nitrospirae,and phylum Gemmatimonadetes. In these,phylum Proteobacteria accounted for 70. 53% of total number of clones,so it was the most dominant group in this library. [Conclusions]The endophytic bacteria diversity in the root of P.dindygulensis Miq. is rich and this study is expected to provide scientific basis for protection,development and use of resources of P. dindygulensis Miq.

关 键 词：PEPEROMIA dindygulensis Miq ENDOPHYTIC BACTERIA Culture-independence DIVERSITY 

分 类 号：S567.219[农业科学—中草药栽培]