荧光定量PCR和ELISA测定乙肝病毒标志物2005例结果分析  

A Comparative Study of Fluorescence Quantitative PCR and ELISA in Detection of HBV Markers

作　　者：陈兆鹏[1] 黄瞻云[1] 张文芳[1] 徐红菊[1] 陈文雨[2] 万海英[1] 

机构地区：[1]同济大学附属同济医院检验科,上海200065 [2]中山大学达安基因诊断中心,广州510089

出　　处：《同济大学学报（医学版）》2003年第2期138-139,143,共3页Journal of Tongji University(Medical Science)

摘　　要：目的　比较FQ PCR和ELISA测定乙肝病毒标志物的相关性。方法　FQ PCR测定HBVDNA ,ELISA测定HBV抗原、抗体。结果　在 2 0 0 5例血清样本中 ,FQ PCR阳性 2 6 4例 ,阳性率 13.1%。其中 12 6例HBeAg阳性者FQ PCR全部阳性 ,阳性率 10 0 %,HBVDNA平均拷贝数 3.1× 10 7;181例HBeAg阴性而HBsAg、HBcAb均阳性。样本FQ PCR132例阳性 ,阳性率 73%,HBVDNA平均拷贝数 2 .6× 10 4 ;8例单项抗HBc、5 90例单项抗HBs、776例乙肝五项指标全部阴性的样本 ,FQ PCR例阳性率分别为 12 .5 %、0 .34 %和 0 .38%。结论　两种方法均具有很好的相关性 ,都是诊断乙肝病毒感染的重要方法。FQ PCR定量测定HBVDNA ,有助于乙型肝炎的早期诊断、疗效观察和预后判断。Objective To study the relationship of HBV DNA and HBV markers in serum.Methods Fluorescence quantitative PCR(FQ-PCR) was used to measure HBV DNA,ELISA was used to detect HBV antigen and antibodies in 2005 cases. Results In 126 HBsAg+ /HBeAg+ /HBcAb+ samples,the FQ-PCR results were all positive with 3.1×10 7/ml of HBV in average.In 181 HBsAg+ /HBeAb+ /HBcAb+ samples,the positive rate of FQ-PCR was 73% with 2.6×10 4 /ml of HBV in average.In 8 samples of HBcAb+,590 samples of HBsAb+ and 776 samples of all HBV antigen and antibodies negative,the positive rate of FQ-PCR were 12.5%,0.34% and 0.38%,respectively.Conclusion The two methods have a good coherence.FQ-PCR which can be used to monitor the true state of HBV infection and amplification,is better in earlier diagnosis and predication of the therapeutic results of hepatitis B.

关 键 词：荧光定量PCR ELISA 测定 乙肝病毒标志物 乙型肝炎 

分 类 号：R512.62[医药卫生—内科学]