变色液体培养基快速检测分枝杆菌的临床应用评价  

A evaluation of rapidly detecting mycobacteria with the colored liquid medium.

作　　者：熊国亮[1] 张慧慧[1] 雷建平[1] 

机构地区：[1]江西省肺科医院检验科,南昌330006

出　　处：《江西医学检验》2003年第2期69-70,106,共3页Jiangxi Journal of Medical Laboratory Sciences

摘　　要：目的对变色液体培养基快速检测分枝杆菌进行评价.方法通过对113例已确诊肺结核病人,24例非结核病人的晨痰分别用一种新型的分支杆菌快速变色液体培养基与改良罗氏培养基进行培养,同时做涂片抗酸染色.结果变色液体培养基阳性率54.9%(62/113),平均检出时间12天(3～17天);改良罗氏培养基阳性率52.2%(59/113);平均报告时间29天(14～42天);两者阳性检出率差异无显著性(x=0.16,P＜0.05).24例非结核病人,痰抗酸染色,改良罗氏培养基,变色液体培养基检测均为阴性结果.结论变色液体培养基检测分支杆菌阳性率高,报告时间短,不需特殊仪器设备,值得在各实验室推广应用.Objective To assess the utility of the colored liquid medium for detecting mycohacteria. Methods The colored liquid medium and the modified Roche medium were used respectively to culture mycohacteria of sputum in 113 tuberculosis and 24 non-tuberculosis patients, and the acid-fast stain was also been performed. Results The positive rate of using the. colored liquid medium was 54.9%(62/l 13), with the, average time for result being 12days(3~17days), while the modified Roche medium, the positive rate was 52.2%(59/l 13), with the time 29 days(14~42days). The results showed no significant difference(.x2=0.16,P>0.05). As to the 24 non-tuberculosis patients, the negative results, were obtained after using the acid-fast slain, the modified Roche medium and the colored liquid medium. Conclusion It is valuable to promote the utility of detecting myeobacteria by the colored liguid medium according to the high positive rate, short time and no requiring for special equipment.

关 键 词：变色液体 培养基 快速检测 分枝杆菌 临床应用 评价 

分 类 号：R378.91[医药卫生—病原生物学] R446.5[医药卫生—基础医学]