人β-NGF基因在CHO细胞中表达的生物学活性及分离纯化  

The study of biological activity of the expression products of the human β-NGF cDNA in CHO cell line and purified the seperated protein

作　　者：白艳艳[1] 杨吉成[1] 缪竞诚[1] 盛伟华[1] 韩晓枫[2] 李丽娥[1] 谢宇锋[1] 周忆新[1] 

机构地区：[1]苏州大学基础医学系细胞免疫中心,苏州215007 [2]无锡市第二人民医院,无锡214002

出　　处：《中国免疫学杂志》2003年第5期343-346,共4页Chinese Journal of Immunology

基　　金：江苏省科委应用基金资助项目 (BJ990 70 )

摘　　要：目的 :检测人 β NGF基因转染CHO细胞后培养上清中所表达的 β NGF的生物学活性及分离纯化。 方法 :采用脂质体介导的真核细胞转染 ,运用形态学观察和MTT法进行检测做定性定量分析 ,蛋白观察用SDS PAGE电泳。结果 :转染 β NGF基因的CHO细胞可分泌能促进PC12细胞生长的活性 β NGF ,且可透过血脑屏障。 结论 :转染 β NGF基因的CHO细胞所分泌的 β NGF ,接近自然合成的蛋白 ,具有较高生物学活性 ,为NGF的生物制药奠定了实验基础。Objective:To detect the biological activity of the expression products of the human β-NGF cDNA in CHO cell line and purified the seperated protein.Methods:Human β-NGF was transfected with lipofectamine reagent into CHO cell line.The biological activity was analyzed by objection with microscope and the method of MTT.The pure protein was proved by SDS-PAGE analysis.Results:The protein in the culture supernatant of the positive CHO cells transfected with β-NGF gene could stimulate the growth of PC12 cell line and go into BBB.Conclusion:The target gene expressed successfully in the transfected CHO cell line and had good biological activity. [

关 键 词：人β-NGF基因 CHO细胞 表达 生物学活性 分离 纯化 

分 类 号：R392－33[医药卫生—免疫学]