ABC-ELISA法诊断肠螨病的研究  

Study on ABC-ELISA in diagnosis of intestinal acariasis

作　　者：黄勇[1] 李朝品[2] 崔玉宝[2] 

机构地区：[1]山东省寄生虫病防治研究所,济宁272033 [2]安徽理工大学医学院

出　　处：《中国人兽共患病杂志》2003年第3期95-97,共3页Chinese Journal of Zoonoses

摘　　要：目的　探讨生物素 -亲和素酶联免疫吸附试验 (ABC -ELISA)在肠螨病诊断中的应用价值。方法　采集 4 8例肠螨病患者血清 ,用ABC -ELISA法检测血清中螨特异性抗体IgG ,并与葡萄球菌A -蛋白酶联免疫吸附试验 (SPA -ELISA)进行比较。结果　用ABC -ELISA法和SPA -ELISA法检测 4 8例肠螨病患者血清中的螨特异性抗体IgG阳性率分别为89 5 8% (43/ 4 8)和 5 6 2 5 % (2 7/ 4 8) ,两者比较 ,差异具显著性 (χ2 =13 5 0 ,P <0 0 1)。结论　ABCAim To explore the value of Avidin Biotin system enzyme linked immunosorbent assay (ABC ELISA) in diagnosis of intestinal acariasis Methods A total of 48 patients with intestinal acariasis were involved in this study ABC ELISA was used to detect the mite specific IgG in serum of the patients,which was compared with SPA ELISA Results The positive rates of the mite specific IgG detected with ABC ELISA and SPA ELISA were 89 58% (43/48) and 56 25% (27/48) respectively The positive rate with ABC ELISA was statistically higher than those with SPA ELISA (χ 2=13 50,P<0 01) Conclusion ABC ELISA is an effective method for diagnosis of intestinal acariasis

关 键 词：ABC-ELISA法 诊断 肠螨病 研究 

分 类 号：R384.4[医药卫生—医学寄生虫学]