β-巯基乙醇诱导成年鼠骨髓基质细胞向神经细胞分化的研究  

Adult Rat Bone Marrow Stromal Cells were Induced Into Neurons by β-Mercaptoethanol

作　　者：赖贤良[1] 邓志锋[1] 汪泱[2] 宋书欣[1] 胡峰[2] 袁铿[3] 胡银英[3] 

机构地区：[1]江西医学院第二附属医院神经外科,江西南昌330006 [2]江西医学院第一附属医院泌尿外科研究所,江西南昌330006 [3]江西省医学科学研究所,江西南昌330006

出　　处：《江西医学院学报》2003年第2期18-20,共3页Acta Academiae Medicinae Jiangxi

基　　金：国家自然科学基金资助项目 (30 16 0 0 84 )

摘　　要：目的 :探讨β 巯基乙醇诱导成年大鼠骨髓基质细胞成神经细胞的可能性。为神经再生和诱导剂的筛选提供一种新的参考方法。方法 :从大鼠骨髓中分离出骨髓基质细胞并培养传至 3- 5代 ,诱导前 2 4h用含 1mmol /l的β 巯基乙醇及含 2 0 %的DMEM培养液培养 ,再以含 5mmol /lβ 巯基乙醇的无血清DMEM培养液作诱导 ,用免疫细胞化学方法鉴定神经细胞特异性标志物NF、NSE及GFAP的表达。结果 :诱导后 1h ,部分细胞发生形态学上的改变 ,6h后大部分细胞不仅在形态上表现为神经元样特征 ,而且NF、NSE等特异性抗体呈阳性表达 ,而GFAP抗体表达呈阴性。结论 :βObjective:To study the possibility thatβ mercaptoethanol induces adult rat bone marrow stromal cells(BMSCs)into neurons for further investigation of an alternative for neurons regeneration and the screening of inducers.Methods:The bone marrow stromal cells were isolated from adult rats and cultured for 3～5 passages.The BMSCs were incubated with DMEM containing 20% FBS and 1 mmol/l β mercaptoethanol (β ME) for 24 hours.Then the media were replaced by DMEM media consisting of 5 mmol/l β mercaptoethanol.The morphological changes of the cells were observed under phase contrast microscope,the cells were stained immunocytochemically with NF,NSE,and GFAP antibodies,respectively.Results:After an hour of induction with β ME,some cells showed morphological changes.Six hours later,many BMSCs became neuron like cells and were positively stained with NSE and NF antibodies,but negatively with GFAP.Conclusion:The bone marrow stromal cells can differentiate into neuron like cells as induction by β mercaptoethanol.

关 键 词：β-巯基乙醇 诱导 成年鼠 骨髓基质细胞 神经细胞 分化 研究 

分 类 号：Q254[生物学—细胞生物学]