β-羟基异戊酰基紫草素对高表达酪氨酸激酶肿瘤细胞系的生长抑制作用(英文)  

Growth inhibitory effects of β-hydroxyisovalerylshi-konin on tumor cells expressing high levels of tyrosine kinases

作　　者：徐颖[1] 王敏伟[1] 中谷一泰 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]昭和大学药学部生物化学教室,日本东京1428555

出　　处：《沈阳药科大学学报》2003年第3期203-206,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的验证 β 羟基异戊酰基紫草素对体外培养的肿瘤细胞的生长抑制作用。 方法XTT比色法。结果低浓度的 β 羟基异戊酰基紫草素抑制人类白血病细胞K5 6 2和U937的生长 ,半数抑制浓度分别是 0 12 μmol/L和 0 14 μmol/L。在较高剂量下 ,β 羟基异戊酰基紫草素抑制酪氨酸激酶高表达细胞系大鼠SR 3Y1和KDR/Flk 1 NIH3T3细胞以及人类表皮细胞癌细胞A4 31的生长。其半数抑制浓度分别是 1 2 μmol/L、2 5 μmol/L、11 0 μmol/L。结论 βObjective To examine the cytotoxic effects of β hydroxyisovalerylshikonin ( β HIVS) on various tumor cells.Method XTT assay was used.Results β HIVS inhibited the growth of leukemia K562 and U937 cells with the estimated IC 50 value of 0 12 μmol/L and 0 14 μmol/L , respectively. At higher concentrations, β HIVS was found to inhibit the cell growth of rat SR 3Y1, KDR/Flk 1 NIH3T3 and human epidermoid A431 cells, which expressed high levels of different species of tyrosine kinases. The estimated IC 50 values for SR 3Y1 cells, for KDR/Flk 1 NIH3T3 cells, and for A431 cells were 1 2 μmol/L , 2 5 μmol/L and 11 0 μmol/L respectively.Conclusion β HIVS has growth inhibitory effect on various tumor cells.

关 键 词：β-羟基异戊酰基紫草素 酪氨酸激酶 细胞毒作用 半数抑制浓度 

分 类 号：R96[医药卫生—药理学]