江浙蝮蛇蛇毒纤溶酶的纯化  

Purification of Fibrinolytic Enzyme from Agkistrodon halyl Venom

作　　者：郑颖[1] 王维祥[1] 向左云[1] 姚光宁[1] 沈居仁[1] 

机构地区：[1]成都军区联勤部军事医学研究所,云南昆明650032

出　　处：《蛇志》2002年第4期7-9,共3页Journal of Snake

摘　　要：目的　从江浙蝮蛇蛇毒中纯化出无出血毒的纤溶酶。　方法　经 DEAE-Sepharose FF离子交换树脂和 Superdex 75 PG凝胶过滤树脂 ,从江浙蝮蛇蛇毒中纯化出 2种可直接溶解纤维蛋白的蛋白酶 :F1 ,F2。　结果　它们 2 0 μg皮下注射小鼠都无出血反应 ,F1 ,F2 SDS-PAGE电泳纯度在 95 %以上。　结论　纯化的两个纤溶酶为进一步基础研究和临床应用奠定了基础。Objective Purificating no hemorrhage of fibrinolytic enzyme from Agkistrodon halyl venom. Methods Using column chromatography separation consisting of DEAE-Sepharose FF ion exchange and Superdex 75 PG gel filtration. Rusult They were no hemorrhage by subcutaneous injection of 20μg F1, F2 to mousses,and the more than 95% on SDS-PAGE, gel electrophoresis. Conclusion This study provided a basis for the further fundamental research and clinical application of fibrinolytic enzyme.

关 键 词：江浙蝮蛇 蛇毒 纤溶酶 纯化 离子交换 凝胶过滤 

分 类 号：R996.3[医药卫生—毒理学]