牛心朴子多糖的提取、分离及其抗S_(180)肉瘤效应初步观察  

EXTRACTION, SEPARATION OF CKPS AND ITSEFFECT FOR ANTI S_(180) SARCOMA

作　　者：刘涛[1] 苏秀兰[2] 李敬福 任靖[1] 

机构地区：[1]内蒙古医学院药学系,内蒙古呼和浩特010059 [2]内蒙古医学院分子生物学中心 [3]内蒙古药品检验所

出　　处：《内蒙古医学院学报》2003年第1期13-16,共4页Acta Academiae Medicinae Neimongol

基　　金：内蒙古教育厅重大科研项目 ( ZD0 1 0 5 1 )

摘　　要：目的 :探讨牛心朴子多糖 ( CKPS)的组成及抗癌活性。方法 :牛心朴子以水 /醇法提取 ,三氯乙酸脱蛋白 ,乙醇沉淀得多糖粗品。继以 sephadex G-2 5纯化牛心朴子粗制多糖 ,完全酸水解后用 TLC及 PC分析组成。采用动物模型考察 CKPS的抑瘤效应。结果 :分离得到一较大量多糖组分 ,该多糖具有一定的抑瘤效应。结论 :牛心朴子多糖为一杂多糖 ,主要由葡萄糖、鼠李糖、木糖、阿拉伯糖和半乳糖组成。体内实验表明 CKPS具一定的抑制 S1 80Objective: To study the composition of CKPS(Cynanchum komarovii Al Iljinski polysaccharide) and its anticancer activity. Methods: Abstracting with water/ethanol system, removing proteins by trichloroacetic acid, obtaining crude polysaccharides with ethanol, refining polysaccharides by sephadexG-25, analyzing its compositions by TLC and PC methods after total acetic hydrolysis. Results: Obtained a kind of polysaccharides. Conclusion: CKPS is a kind of heteropolysaccharide containing glucose, rhamnose, xylose, arabinose, galactose. The experiments in vivo displayed that CKPS has the inhibiting growth effect of S 180 sarcoma cell.

关 键 词：牛心朴子 提取分离 S180肉瘤 

分 类 号：R965.1[医药卫生—药理学] R931.71[医药卫生—药学]