青蒿提取物抗单纯疱疹病毒活性研究  

A STUDY OF ANTIVIRAL ACTIVITY AGAINST HSV-2 OF THE EXTRACT FROM ARTEMISIA ANNUA L

作　　者：张军峰[1] 谭健[1] 蒲蔷[1] 刘颖华[1] 何开泽[1] 

机构地区：[1]中国科学院成都生物研究所,四川成都610041

出　　处：《天然产物研究与开发》2003年第2期104-108,共5页Natural Product Research and Development

摘　　要：用细胞病变效应 (CPE)法证明了青蒿水提物具有抗单纯疱疹病毒Ⅱ型 (HSV 2 )活性 ,通过初步分离纯化得到抗HSV 2活性的有效成分。用MTT法研究了青蒿水提物和有效成分的细胞毒性和抗HSV 2活性 ,CC50 分别为 5 2 9mg/ml和 4 94mg/ml,IC50 分别为 1 4 5mg/ml和 0 12 8mg/ml,TI分别为 3 6 5和 38 6。以 0 5mg/ml的无环鸟苷 (ACV)作为阳性对照 ,结果显示有效成分在体外可以明显抑制HSV - 2的致细胞病变作用 ,效果与ACV相当。The anti herpes simplex virus type 2 activity of the crude water extract of Artemisia annua L.was proved by cytopathogenic effect(CPE)method.By re extracting with different solvents,the partially separated components maintaining antiviral activity were obtained.The 50% cytotoxity concentration(CC 50 ),50% inhibition concentration(IC 50 )and therapy index(TI)of the crude water extract and the partially separated components were 4 94 mg/ml,0 128 mg/ml,38 6 and 5 29 mg/ml,1 45 mg/ml,3 65,respectively measured by MTT method with 0 5 mg/ml of ACV as the positive control,showing that the partially separated components have remarkable anti HSV 2 activity in vitro.

关 键 词：青蒿提取物 单纯疱疹病毒 抗病毒活性 细胞病变效应 有效成分 细胞毒性 

分 类 号：R282.71[医药卫生—中药学]