苯那普利对糖尿病大鼠肾脏细胞凋亡及Fas和Fas-L表达的影响  

Effects of benazepril on apoptosis and expression of Fas and Fas-L in the kidney of diabetic rats

作　　者：张新爽[1] 刘亚玲 张艳玲[3] 

机构地区：[1]河北省衡水市中医院,053000 [2]邯郸钢铁公司职工医院 [3]河北医科大学第三医院

出　　处：《河北医药》2003年第4期243-245,共3页Hebei Medical Journal

摘　　要：目的　探讨血管紧张素转换酶抑制剂 (ACEI)对糖尿病肾脏细胞凋亡及凋亡相关蛋白的影响。方法　单侧肾切除大鼠腹腔注射STZ(65mg kg)诱发糖尿病模型 ,每日灌胃给予ACEI苯那普利(10mg kg) ,共 12周。采用原位末端标记法检测肾脏细胞凋亡情况 ,流式细胞术和免疫组化检测肾皮质Fas和Fas L表达。结果　糖尿病组较对照组肾小球、肾小管凋亡细胞数明显增多 ,Fas和Fas L的表达亦显著增强 ,苯那普利治疗组较糖尿病组凋亡细胞数减少 ,Fas和Fas L的表达减弱。结论　苯那普利可能通过影响凋亡相关蛋白Fas和Fas L的表达而抑制肾脏细胞凋亡 ,从而发挥肾脏保护作用。Objective To investigate the effects of angiotensin converting enzyme inhibitor on apoptosis and the expression of Fas and Fas L in the kidney of diabetic rats.Methods Uninephrectomized SD rats were used to induce diabetes by intraperitoneal injection of STZ(65 mg/kg).Benazepril(10 mg/kg) was delivered daily by gavage from the next day of the induction to diabetes for 12 weeks.Apoptosis was evaluated by means of terminal deoxynucleotidyl transferase mediated d UTP nick end labeling (TUNEL).Flow cytometry and immunohistochemistry were used to detect the expression of Fas and Fas L.Results Compared with those of the kidneys in control group,apoptotic cells were more in number and the expression of Fas and Fas L was higher in the diabetic kidneys (P<0.05).The number of apoptotic cells and the level of expression of Fas and Fas L were reduced by benazepril treatment (P<0.05).Conclusion Angiotensin converting enzyme inhibitor benazepril has some renal protective effect on diabetic nephropathy,partly through inhibiting apoptosis by down-regulating Fas and Fas L expression.

关 键 词：糖尿病肾病 血管紧张素转换酶抑制剂 苯那普利 细胞凋亡 FAS FAS-L 

分 类 号：R587.2[医药卫生—内分泌]